Mingda Yin^1,#, Rui Luo^1,#, Tana Liang¹, Qi Wen¹, Xiaotian Liang¹, Yanpeng Wen¹, Xuemei Hu¹, Zhiyan Wang¹, Chang Gao¹, Wenjing Ren¹, Fenglan Huang^1,2,3,4,5,*

Phyton-International Journal of Experimental Botany, Vol.93, No.1, pp. 97-117, 2024, DOI:10.32604/phyton.2023.031228 - 26 January 2024

Abstract Castor is one of the top 10 oil crops in the world and has extremely valuable uses. Castor inflorescences directly affect yield, so the study of inflorescence development is very important in increasing castor yield. Our previous studies have shown that the PIP5K gene family (PIP5Ks) is associated with inflorescence development. In this study, to determine the function of each PIP5K gene in castor, a female Lm-type castor line, aLmAB2, was used to determine the relative expression levels of the PIP5Ks in castor inflorescences. Six PIP5K genes were heterologously overexpressed in Arabidopsis thaliana, the relative expression of each gene… More >

TINGTING HUANG^1,#, SHAN DENG^2,#, JIANGYUAN SHENG¹, DI ZHANG^1,*

BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314 - 07 July 2022

Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK₃ type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE… More >