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    ARTICLE

    Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

    Yulin Luo1,2,#, Panpan Yang2,#, Mengmeng Bi2, Leifeng Xu2, Fang Du3,*, Jun Ming2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 1057-1066, 2024, DOI:10.32604/phyton.2024.051197

    Abstract The xylem undergoes physiological changes in response to various environmental conditions during the process of plant growth. To understand these physiological changes, it is extremely important to observe the transport of xylem. In this study, the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin section. Methods for introducing a fluorescent tracer into the xylem of the stems were evaluated. Then, the transport rule of 5(6)-Carboxyfluorescein diacetate (CFDA) in the xylem of the stem of L. lancifolium was studied by fluorescence dye in live cells tracer technology. The results showed… More >

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