WANG JIANMIN, SONG RUIHUA, CHEN LEI, YIN LIANGJUN, CHEN BO, SUN JING, GOU YUANBING, ZHAO LING, CHEN LIN
BIOCELL, Vol.30, No.2, pp. 269-278, 2006, DOI:10.32604/biocell.2006.30.269
Abstract Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were… More >
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