Haijian Yang¹, Mingzhi Zhang^1,*, Na Xiao¹, Yuan Li²

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1543-1568, 2024, DOI:10.32604/phyton.2024.050915 - 30 July 2024

Abstract The micro-sprinkler irrigation mulched (MSM) has been suggested as a novel water-saving approach in controlled environment agriculture. However, the effects of microbial community structure and enzyme activity in the rhizosphere soil on crop growth under MSM remain unclear. This study conducted a randomized experimental design using greenhouse tomatoes to investigate changes in bacterial community structure and enzyme activity in rhizosphere soil under different irrigation frequencies (F) and amounts (I) of MSM. The findings revealed that with the increase of F or I, The total count of soil bacteria in tomatoes first rose and then fell… More >

Dejin Mu^1,2, Lin Chen^1,2, Heze Wang^1,2, Zhaoliu Hu^1,2, Sihui Chen^1,2, Shi Chen^1,2, Nianhui Cai^1,2, Yulan Xu^1,2, Junrong Tang^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 503-516, 2024, DOI:10.32604/phyton.2024.048786 - 28 March 2024

Abstract Phenylalanine ammonia lyase (PAL) is the rate-limiting and pivotal enzyme of the general phenylpropanoid pathway, but few reports have been found on PAL genes in Pinus yunnanensis. In the present study, three PAL genes were cloned and identified from P. yunnanensis seedlings for the first time, namely, PyPAL-1, PyPAL-2, and PyPAL-3. Our results indicated that the open-reading frames of PyPAL genes were 2184, 2157, and 2385 bp. Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants. In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form More >

Yajun Li, Yihui Wang, Shuang Chen, Yu Gao, Yan Shi^*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2151-2164, 2023, DOI:10.32604/phyton.2023.027930 - 29 May 2023

Abstract

In recent years, in order to improve nutrient use efficiency, especially nitrogen use efficiency, fertilizer value-added technology has been developed rapidly. However, the mechanism of the effect of synergistic fertilizer on plant nitrogen utilization is not clear. A study was, therefore, conducted to explore the activities and gene expression of key enzymes for nitrogen assimilation and the gene expression of nitrogen transporters in wheat after the application of synergistic fertilizer. Soil column experiment was set up in Qingdao Agricultural University experimental base from October 2018 to June 2019. Maleic acid and itaconic acid were copolymerized with

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Sareh HAJIABADI¹, Mansour MASHREGHI¹, Ahmad Reza BAHRAMI^1,2, Kiarash GHAZVINI³, Maryam M. MATIN^1,2,*

BIOCELL, Vol.44, No.1, pp. 63-71, 2020, DOI:10.32604/biocell.2020.08171 - 01 March 2020

Abstract Cellulose is the main structural component of lignocellulosic wastes that can be converted to sugars and biofuels by cellulase. Due to wide applications of this enzyme in various industries around the world, cellulase is considered as the third industrial enzyme. The ability of thermophilic bacteria in the production of heat-stable cellulases has made them valuable tools in biotechnology. The aim of this study was isolation and molecular identification of cellulolytic thermophile bacteria from Dig Rostam hot spring and investigating their cellulase activity. Samples were taken from water and sediments of this hot spring, and cellulolytic… More >

Abrameto MA, CM Pozzo Ardizzi, MI Gil, LM Molina

Phyton-International Journal of Experimental Botany, Vol.79, pp. 123-132, 2010, DOI:10.32604/phyton.2010.79.123

Abstract Costly investments in storage and transport of onion bulbs makes it increasingly important to identify cultivars with the best chance of long-term storage. This paper discusses our own and other researchers’ results and laboratory procedures on onion’s pungency, soluble solids, dry matter, respiration rate, carbohydrates and catabolism-related enzymes under different storage conditions. The variability in parameters such as carbohydrate composition, pungency, soluble solids and soluble uronic acid concentration in water is growth dependent, and defines the quality of onion bulbs at harvest time. During storage, these parameters can be modified by environmental conditions, presence of More >