OSCAR MAC DONAL1, JUAN G. CHEDIACK1,2,3, ENRIQUE CAVIEDES-VIDAL1,2,3
BIOCELL, Vol.32, No.3, pp. 219-227, 2008, DOI:10.32604/biocell.2008.32.219
Abstract The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi More >
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