B.C. Heng*, K.J. Vinoth*, K. Lu*, X. Deng*, Z. Ge*, B.H. Bay**, T. Cao*
BIOCELL, Vol.31, No.3, pp. 405-410, 2007, DOI:10.32604/biocell.2007.31.405
Abstract We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin… More >
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