Chunxue Peng1,2, Fang Gao1,2, Hao Wang1,2, Iraida Nikolaevna Tretyakova3, Alexander Mikhaylovich Nosov4,5, Hailong Shen1,2,*, Ling Yang1,2,*
Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 223-238, 2022, DOI:10.32604/phyton.2022.015523
- 16 August 2021
Abstract Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR)… More >
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