Katerina Ondraskova1,2, Matous Cwik3, Ondrej Horky4, Jitka Berkovcova4, Jitka Holcakova1, Martin Bartosik1, Tomas Kazda5, Klara Mrazova1,6, Michal Uher7, Igor Kiss3, Roman Hrstka1,3,*
Oncology Research, Vol.34, No.2, 2026, DOI:10.32604/or.2025.070116
- 19 January 2026
Abstract Objectives: Cancer treatment relies heavily on accurate diagnosis and effective monitoring of the disease. These processes often involve invasive procedures, such as colonoscopy, to detect malignant tissues, followed by molecular analyses to determine relevant biomarkers. This study aimed to evaluate the clinical performance of droplet digital PCR (ddPCR) for detecting Kirsten Rat Sarcoma Viral Proto-Oncogene (KRAS), Neuroblastoma RAS Viral Oncogene Homolog (NRAS), and B-Raf Murine Sarcoma Viral Oncogene Homolog B (BRAF) mutations in circulating tumor DNA (ctDNA) from colorectal cancer patients using liquid biopsy. Methods: ctDNA was isolated from colorectal cancer (CRC) patients (n = 110) and analyzed for KRAS, BRAF,… More >