Zhanwu Hou¹, Linfeng Xu^2,*

The International Conference on Computational & Experimental Engineering and Sciences, Vol.30, No.1, pp. 1-0, 2024, DOI:10.32604/icces.2024.012892

Abstract The cell-cell interaction between immune cells and tumor cells in the tumor microenvironment plays an important role in the genesis and development of tumors. However, due to the lack of methods to systematically identify the interaction between the two, the specific molecular mechanisms involved are not well understood. The microfluidic platform provides a high-throughput and precise method for studying cell interactions in microreactive systems. However, the traditional platform for studying cell interactions is the closed droplet system, which is easy to cause the consumption of nutrients and the accumulation of wastes, thus interfering with cell… More >

SAMANEH MOSTAFAVI, ZUHAIR MOHAMMAD HASSAN^*

Oncology Research, Vol.32, No.3, pp. 477-487, 2024, DOI:10.32604/or.2023.043926 - 06 February 2024

Abstract Intracellular communications between breast cancer and fibroblast cells were reported to be involved in cancer proliferation, growth, and therapy resistance. The hallmarks of cancer-fibroblast interactions, consisting of caveolin 1 (Cav1) and mono-carboxylate transporter 4 (MCT4) (metabolic coupling markers), along with IL-6, TGFβ, and lactate secretion, are considered robust biomarkers predicting recurrence and metastasis. In order to promote a novel phenotype in normal fibroblasts, we predicted that breast cancer cells could be able to cause loss of Cav1 and increase of MCT4, as well as elevate IL-6 and TGFβ in nearby normal fibroblasts. We created a… More >

Fatemeh Bayati^1,*, Maryam Valadi^1,*, Armin Ahmadi^1,2, Farangis Najaf¹, Bita Ansaripour¹, Ehsan Sharif-Paghaleh^1,3

European Cytokine Network, Vol.33, No.3, pp. 70-78, 2022, DOI:10.1684/ecn.2022.0482

Abstract Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant More >

Patrycja Libako¹, Julia Miller¹, Wojciech Nowacki¹, Sara Castiglioni², Jeanette A. Maier², Andrzej Mazur³

European Cytokine Network, Vol.26, No.1, pp. 1-9, 2015, DOI:10.1684/ecn.2015.0361

Abstract Magnesium is highly involved in the metabolic network such that even subtle disturbances in its homeostasis affect many cellular functions, including calcium homeostasis, signal transduction, energy metabolism, membrane stability and cell proliferation. Recently, magnesium level has been proposed to modulate the priming and activity of immune cells. We studied the behavior of antigen-presenting cells (APCs) and T lymphocytes after altering the magnesium/calcium balance. We used two different populations of primary APCs, i.e. bone marrow-derived dendritic cells and bone marrow-derived macrophages, while D10.G4.1 cells served as a model of responding Th2 cells. Our principal findings are More >

Aleksandra Krstić¹, Marija Vlaski^2,3, Mohammad Hammoud², Jean Chevaleyre², Pascale Duchez², Gordana Jovčić¹, Diana Bugarski¹, Pavle Milenković¹, Philippe Bourin⁴, Jean-Michel Boiron^2,3, Vincent Praloran^3,5, Zoran Ivanović²

European Cytokine Network, Vol.20, No.1, pp. 10-16, 2009, DOI:10.1684/ecn.2009.0145

Abstract Co-culture of haematopoietic cells with a stromal cell layer does not mimic the physiological, micro-environmental niche, whose major feature is a low oxygen (O₂) concentration. Thus, in order to study the effects of IL-17 in a context which better approximates the physiological state, we investigated its effects on cell expansion, colony-forming ability, and the phenotypical profile of normal, human blood CD34⁺ cells co-cultured for five days with MSC layers at various O₂ concentrations (20%, 12.5% and 3% O₂). We demon-strated that IL-17 enhances CD34⁺ and total CFC production during the five days of MSC/CD34⁺ co-culture. This effect depends More >