Xin Qi^1,#, Yu Wang^1,#, Xing Zhang¹, Xiaoshuang Wei¹, Xinyang Liu¹, Zhennan Wang¹, Zhenhui Wang^1,*, Fenglou Ling^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1767-1779, 2024, DOI:10.32604/phyton.2024.052844 - 30 July 2024

Abstract DNA methylation is an important epigenetic regulatory mechanism, it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. In this study, a novel methyltransferase 2a gene (Zmet2a) was cloned in maize and identified by polymerase chain reaction-base (PCR-base) using a bioinformatics strategy. The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides. The Zmet2a protein revealed that it contains BAH and CHROMO structural domains, is a non-transmembrane protein that is hydrophilically unstable, and has no signal peptide structure. Meanwhile, we verified More >

Xiaokui Huang¹, Yingbin Xue¹, Aaqil Khan¹, Hanqiao Hu¹, Naijie Feng^1,2,*, Dianfeng Zheng^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2369-2382, 2023, DOI:10.32604/phyton.2023.027457 - 25 June 2023

Abstract For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure. More >

ZHENGHONG FENG¹, BING GAO¹, YU GAO², JIANHUI WU^1,*

BIOCELL, Vol.47, No.7, pp. 1571-1582, 2023, DOI:10.32604/biocell.2023.029106 - 21 June 2023

Abstract Background:Potentilla sericea is a heavy metal hyperaccumulator landscaping plant. MYB transcription factors play an important role in regulating plant stress response to adversity. However, there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea. In this study, the gene was successfully cloned from Potentilla sericea. Methods: Bioinformatic analysis and real-time quantitative PCR (qPCR) methods were used to evaluate this gene. The transgenic A. thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress. Results: The open reading frame of is 942 bp, which encodes 313 amino… More >

Jiali Wang¹, Xiangmei Chen¹, Xiaozhen Huang¹, Yichen Zhao^1,*, Degang Zhao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1377-1389, 2023, DOI:10.32604/phyton.2023.026830 - 09 March 2023

Abstract The phytoene desaturase (PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting PDS expression leads to the appearance of mottled, chlorosis, or albino leaves. In this study, the CDS sequence of EuPDS (Eucommia ulmoides Phytoene Desaturase) was first cloned and then PDS was silenced in Nicotiana benthamiana. Result showed the expression level of EuPDS in leaves was higher than that in the roots and stems. In N. benthamiana leaves, which were treated by Agrobacterium for 24 h, photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the More >

Maria Babar¹, Siddra Ijaz^1,*, Imran Ul Haq², Muhammad Sarwar Khan¹

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469 - 06 January 2023

Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of… More >

Shuo Qu¹, Qi Cai², Huimin Cui¹, Lamboro Abraham¹, Yaolei Jiao¹, Guilong Ma², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 501-519, 2023, DOI:10.32604/phyton.2022.023479 - 12 October 2022

Abstract High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil… More >

Xiaofang Zeng^1,#, Guangzheng Li^1,#, Nu’an Liu², Yan Li¹, Jianrong Li¹, Xiaozhen Huang¹, Degang Zhao^1,2,,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2419-2437, 2022, DOI:10.32604/phyton.2022.021199 - 12 July 2022

Abstract Leaf-color mutants play an important role in the study of chlorophyll metabolism, chloroplast development, and photosynthesis system. In this study, the yellow leaf 1 (yl1) rice mutant was identified from the ethyl methane sulfonate-treated mutant progeny of Lailong, a glutinous japonica rice landrace cultivated in Guizhou Province, China. Results showed that yl1 exhibited yellow leaves with decreased chlorophyll content throughout the growth period. Chloroplast development in the yl1 mutant was disrupted, and the grana lamellae was loosely packed and disordered. RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) analysis revealed that the chlorophyll synthesis-related genes OsCHLH, OsCHLM, OsCHLG, PORB,… More >

Siyan Liu^1,*, Jinfeng Liu¹, Yuzhe Zhang¹, Yushi Jiang¹, Shaowang Hu¹, Andi Shi¹, Qiyao Cong¹, Shuyan Guan^2,*, Jing Qu², Yao Dan¹

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1465-1482, 2022, DOI:10.32604/phyton.2022.018836 - 14 March 2022

Abstract Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were… More >

Yuzhe Zhang^1,#, Guanglong Li^1,#, Shaowang Hu¹, Jinfeng Liu¹, Yushi Jiang¹, Siyan Liu^2,*, Shuyan Guan^2,*, Jing Qu², Dan Yao¹, Andi Shi¹, Yixuan Liu¹

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1183-1198, 2022, DOI:10.32604/phyton.2022.018853 - 14 February 2022

Abstract Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance… More >

Yulu Chen¹, Fen Zhang¹, Ju Cai¹, Yichen Zhao^1,2, Jiaxue Cui¹, Yan Li^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 999-1013, 2022, DOI:10.32604/phyton.2022.018984 - 24 January 2022

Abstract Gibberellic acid controlled the key developmental processes of the life cycle of landing plants, and regulated the growth and development of plants. In this study, a novel gibberellin receptor gene EuGID1 was obtained from Eucommia ulmoides Oliver. The cDNA of EuGID1 was 1556 bp, and the open reading frame was 1029 bp, which encoded 343 amino acids. EuGID1 had the homology sequence with the hormone-sensitive lipase family. Amino acid sequence alignment confirmed EuGID1 protein had the highest homology with the GID1 protein of Manihot esculenta. EuGID1 was located in the nucleus and cell membrane and had expression in four plant More >