Wentao Sheng, Xi Lei, Xinjie Chen, Quan Kuang^*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1897-1911, 2024, DOI:10.32604/phyton.2024.052395 - 30 August 2024

Abstract The analysis of chloroplast gene characteristics in Alpinia japonica (Thunb.) Miq. is of great significance for developing relevant genetic resources. The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A. japonica. The total chloroplast genome length of A. japonica was 161,906 bp, with a typical circular tetrameric structure. And 133 genes were annotated, comprising 87 protein-coding, 38 tRNA, and 8 rRNA genes. Furthermore, 22 genes contained two copies, and 18 genes owned introns. Repeat sequence analysis showed that it contains 321 simple sequence repeats (SSRs) and 37 long segment repeats. Compared with… More >

Riwa Mahai¹, Rongpeng Liu¹, Xiaolang Du¹, Zejing Mu¹, Xiaoyun Wang^1,*, Jun Yuan^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1203-1219, 2024, DOI:10.32604/phyton.2024.051559 - 27 June 2024

Abstract Rosaceae represents a vast and complex group of species, with its classification being intricate and contentious. The taxonomic placement of many species within this family has been a subject of ongoing debate. The study utilized the Illumina platform to sequence 19 plant species from 10 genera in the Rosaceae. The cp genomes, varying in size from 153,366 to 159,895 bp, followed the typical quadripartite organization consisting of a large single-copy (LSC) region (84,545 to 87,883 bp), a small single-copy (SSC) region (18,174 to 19,259 bp), and a pair of inverted repeat (IR) regions (25,310 to… More >

XIAOJUN ZHOU^1,*, MENGXUE LIU¹, LINLIN SONG²

BIOCELL, Vol.47, No.3, pp. 657-668, 2023, DOI:10.32604/biocell.2023.026781 - 03 January 2023

Abstract Azalea is a general designation of Rhododendron in the Ericaceae family. Rhododendron not only has high ornamental value but also has application value in ecological protection, medicine, and scientific research. In this study, we used Illumina and PacBio sequencing to assemble and annotate the entire chloroplast genomes (cp genomes) of four Rhododendron species. The chloroplast genomes of R. concinnum, R. henanense subsp. lingbaoense, R. micranthum, and R. simsii were assembled into 207,236, 208,015, 207,233, and 206,912 bp, respectively. All chloroplast genomes contain eight rRNA genes, with either 88 or 89 protein-coding genes. The four cp genomes were compared and analyzed by More >

Sisi Liu¹, Lisi Zhou², Jiaoli Huang¹, Huijie Zeng¹, Zhongquan Qiao¹, Yongxin Li^1,*, Gang Zhang^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1503-1516, 2022, DOI:10.32604/phyton.2022.020390 - 14 March 2022

Abstract Lonicerae Flos (LF) derived from the dried flower buds or opening flowers of four Lonicera plants (Lonicera macranthoides, L. hypoglauca, L. confusa, and L. fulvotnetosa), is a popular traditional Chinese medicine. Because the four origin plants are very similar in morphology, it is difficult to control the quality of LF in actual production. Over the past decade, many reports have pointed out the differences among them, including the botanical characteristics and active ingredients. However, there is still a lack of rapid methods that can be applied to the identification of the four origins. In this study, comparative analysis of the… More >

WAN ZHANG^1,2,#, GUIYAN YANG^3,#, YUNLIN ZHAO¹, ZHENGGANG XU^1,2,3,*, HUANG HUIMIN¹, JIAKANG ZHOU¹

BIOCELL, Vol.46, No.3, pp. 803-819, 2022, DOI:10.32604/biocell.2022.016984 - 18 November 2021

Abstract Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp More >

Yingting Zhang^1,2,3, Junjie Yang^1,2,3, Zhenhao Guo^1,2,3, Jiaxing Mo^1,2,3, Jiebing Cui^1,2,3, Hailiang Hu^1,2,3, Jin Xu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 957-986, 2020, DOI:10.32604/phyton.2020.011211 - 09 November 2020

Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28… More >

Yuhao Weng¹, Liming Zhu², Yan Ma³, Hao Su¹, Lu Lu¹, Pengkai Wang¹, Jinhui Chen¹, Asif Ali¹, Renhua Zheng^4,*, Jisen Shi^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 375-384, 2020, DOI:10.32604/phyton.2020.08831 - 22 April 2020

Abstract Cerasus Campanulata is one of several species belonging to the Prunoideae focke, a subfamily of the flowering plant Rosaceae. We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination. Here, we determined the complete chloroplast (cp) genome sequence of C. campanulata and performed sequence analysis to reveal the presence of 18 forward repeats, 20 palindrome repeats, 2 complement repeats, 4 reverse repeats and 93 simple sequence repeats (SSRs). We additionally performed a comparative study of C. campanulata and seven other Prunoideae focke species. Then, maximum parsimony (MP) and maximum likelihood (ML) More >