Tatiana M. Astakhova, Nikita S. Karpov, Nataliya O. Dashenkova, Elena V. Alpeeva, Mikhail V. Nesterchuk, Sergey B. Akopov, Arsen S. Mikaelyan, Anfisa S. Ryabchenko, Pavel A. Erokhov, Natalia P. Sharova^*

Oncology Research, Vol.33, No.9, pp. 2573-2595, 2025, DOI:10.32604/or.2025.066611 - 28 August 2025

Abstract Objectives: Proteasomes, multi-subunit proteases, are key actors of cellular protein catabolism and a number of regulatory processes. The detection of subtle proteasome functioning in tumors may contribute to our understanding of the mechanisms of cancer development. The current study aimed to identify the role of low molecular mass protein 2 (LMP2), a proteasome immune subunit, in the development of mouse colon 26 (C26) adenocarcinoma. Methods: The functions of the LMP2 subunit in tumor development in Balb/c mice were studied using its irreversible inhibitor KZR-504. LMP2 activity was detected by the hydrolysis of the fluorogenic substrate… More >

Alexis Salazar-Navarro¹, Víctor Ruíz-Valdiviezo², José Joya-Dávila¹, Daniel González-Mendoza^1,*, Olivia Tzintzun-Camacho¹, Ulin Basilio-Cortes¹, Dagoberto Duran-Hernández¹, Henry López-Lopez¹, Onecimo Grimaldo-Juárez¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 641-655, 2025, DOI:10.32604/phyton.2025.062871 - 31 March 2025

Abstract The biochemical response of Coffea arabica var. Borbon to chitosan and chitosan oligosaccharides (COS) was evaluated in one-year-old plants under greenhouse conditions. COS solutions were synthesized through chemical and physical hydrolysis using acetic acid, hydrogen peroxide, and microwave irradiation. The obtained COS had an average molecular weight (Mw) of 3549.90 ± 0.33 Daltons (Da), a deacetylation degree (DD) of 76.64 ± 1.12%, and a polymerization degree (PD) of 18.91 ± 0.0018. Solutions of chitosan and COS were applied to C. arabica var. Borbon at concentrations of 0.25, 0.5, and 1 wt%. The experimental design was conducted using… More >

Lanjun Li¹, Degang Zhao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3043-3061, 2023, DOI:10.32604/phyton.2023.031175 - 24 October 2023

Abstract Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with… More >

José Adrian Perez-Robles, Carlos Alberto Lecona-Guzmán^*, Víctor Manuel Ruíz-Valdiviezo, Joaquín Adolfo Montes-Molina^*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2503-2518, 2023, DOI:10.32604/phyton.2023.029784 - 28 July 2023

Abstract The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L. infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts. Solanum lycopersicum plantlets were transplanted 15 days after the emergency. Five days after transplanting, Beauveria bassiana spores were applied at a concentration of 1 × 10⁷ spores mL⁻¹ onto soil (along with A. indica (N) and P. auritum (H) leaf extracts) where S. lycopersicum plants were planted. Eight days after transplanting, spores of F. oxysporum strain were applied at a concentration of 1 × 10⁶ spores mL⁻¹ to soil where S. lycopersicum plants were growing.… More >

Henry López-López¹, Nancy Ruiz-Lau², Rocío Meza-Gordillo¹, Víctor Manuel Ruiz-Valdiviezo¹, Joseph Galthier Robledo-Luchetti¹, Carlos Alberto Lecona-Guzmán¹, Juan José Villalobos-Maldonado¹, Luc Dendooven³, Joaquín Adolfo Montes-Molina^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1235-1255, 2023, DOI:10.32604/phyton.2023.025716 - 06 January 2023

Abstract The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 10⁸ conidia·mL⁻¹, which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 10⁶ spores·mL⁻¹, which were added directly to the soil. Spectrophotometry was used for measuring agronomic… More >

González-Franco AC¹, L Robles-Hernández¹, JL Strap²

Phyton-International Journal of Experimental Botany, Vol.86, pp. 14-27, 2017, DOI:10.32604/phyton.2017.86.014

Abstract The Streptomyces genus comprises a large and diverse group of bacteria, many of which are commercially exploited for the production of antibiotics and lytic enzymes. The thermophilic species are less studied than the predominant mesophilic species. However, the first ones are a potential source of thermostable bioactive products and enzymes with novel properties. In this study, two selected thermophilic streptomycetes were identified and their chitinolytic activities were evaluated. The identification of these two isolates was performed by microscopic morphology, partial 16S rDNA sequences, and its phylogenetic analysis. To study the chitinolytic activities of these isolates, the… More >

Yan PM¹, HF Zhang¹, Q Wang¹, XY Yan¹, Y Sun²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 117-121, 2010, DOI:10.32604/phyton.2010.79.117

Abstract Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud… More >