Xuesong Wang1,#, Zhihong Wang1,2,#, Qiuling Yang1, Sheng Peng3, Mijun Peng1,*
Journal of Renewable Materials, Vol.10, No.12, pp. 3131-3145, 2022, DOI:10.32604/jrm.2022.020204
- 14 July 2022
Abstract To evaluate the potential anti-aging ability of Eucommia ulmoides, four characteristic components (chlorogenic
acid, geniposidic acid, aucubin, quercetin) were selected to assess their effects on H2O2-induced oxidative damage
model of human umbilical vein endothelial cell (HUVEC). Oxidative damage indexes, inflammatory factors, cell
cycle, cell apoptosis, cell senescence, and their related proteins were analyzed by methyl thiazolyl tetrazolium
(MTT) assay, enzyme-linked immunosorbent assay (ELISA), propidium iodide (PI) staining, annexin V-FITC/
PI double staining, SA β-galactosidase staining, and western blotting (WB). The results showed that H2O2-
induced cell growth inhibition rate decreased as supplementation with characteristic components when… More >
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