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    ARTICLE

    Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers

    ZONG-YUN LI1,2*, MEI-LI FU1, FANG-FANG HU1, SHU-FENG HUANG1, YUN-CHUN SONG2

    BIOCELL, Vol.30, No.1, pp. 27-32, 2006, DOI:10.32604/biocell.2006.30.027

    Abstract The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual More >

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