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  • Open Access

    ARTICLE

    High Throughput Image Analysis between Seed Traits Opens New Breeding Avenues in Tartary Buckwheat Germplasm

    Bo Hwan Kim1,#, Sheikh Mansoor2,#, Gyung Deok Han3, Ji Eun Park4, Wook Kim1,*, Yong Suk Chung2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2339-2347, 2024, DOI:10.32604/phyton.2024.056439 - 30 September 2024

    Abstract Recognizing the variation of genetic resources is the first step in selection. One of the most important variations in grain crops is the uniformity of seed grain weight, which can be converted into seed size. However, it has been challenging since it needs high labor costs and time to measure it on a large scale. The current study used an image analysis technique to measure the grain seed area of about 100 seeds per accession with 64 germplasm of Tartary buckwheat (Fagopyrum tataricum) to study variation among and within them. To understand the nature of variation, More >

  • Open Access

    REVIEW

    Application of Transgenic Technology in Identification for Gene Function on Grasses

    Lijun Zhang, Ying Liu*, Yushou Ma*, Xinyou Wang

    Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1913-1941, 2024, DOI:10.32604/phyton.2024.052621 - 30 August 2024

    Abstract Perennial grasses have developed intricate mechanisms to adapt to diverse environments, enabling their resistance to various biotic and abiotic stressors. These mechanisms arise from strong natural selection that contributes to enhancing the adaptation of forage plants to various stress conditions. Methods such as antisense RNA technology, CRISPR/Cas9 screening, virus-induced gene silencing, and transgenic technology, are commonly utilized for investigating the stress response functionalities of grass genes in both warm-season and cool-season varieties. This review focuses on the functional identification of stress-resistance genes and regulatory elements in grasses. It synthesizes recent studies on mining functional genes, regulatory More >

  • Open Access

    ARTICLE

    Effects of Flowering Mode and Pollinator Sharing on Reproductive Success in Natural Hybrid of Two Epimedium (Berberidaceae) Species

    Lanying Chen1,2, Qiumei Quan3,*, Yunxiang Li3

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 551-566, 2024, DOI:10.32604/phyton.2024.048103 - 28 March 2024

    Abstract Mediated by pollen flow, natural hybridization is deemed a crucial factor that propels speciation in floral plants. Despite the fact that the diversity of Epimedium species is concentrated mainly in Southwestern China, the potential impact of interspecific pollination on natural hybridization has yet to be empirically scrutinized. To explore this, we studied the flowering period and the visitors of flowers in three frequently overlapping Epimedium species at Jinchengshan National Forest Park, located in Nanchong, Sichuan Province. Additionally, we performed a series of pollination experiments to examine breeding systems and hybrid compatibility. Morphologically, Epimedium pubescens and Epimedium wushanense are… More >

  • Open Access

    ARTICLE

    Pollination Biology of the Endangered Herbal Medicines Dendrobium chrysotoxum (Orchidaceae)

    Kai Luo1, Yanbing Bai1, Yan Jiang1, Guiling Li1, Min Jia1, Yongguo He1, Zhijun Yao1, Ziwei Xiao1, Xiaoqiong Liu1, Wenjie Liu2, Shanshan Yin1,*, Zesheng Li1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 1975-1986, 2023, DOI:10.32604/phyton.2023.028232 - 29 May 2023

    Abstract Pollination biology studies of the endangered herbal medicines Dendrobium chrysotoxum were conducted in natural pollination conditions using flower observation, pollinator observation and artificial pollination experiments. Populations of D. chrysotoxum with fragrance and nectar were pollinated by Ctenoplectra davidi Valhalla (Hymenoptera: Apidae) species. The floral structure of D. chrysotoxum adapted precisely to its pollinators. Flowers had a low capsule setting (0.17%) under natural conditions. However, compared to open pollination, artificial pollination experiments showed a significant increase in capsule setting, and D. chrysotoxum was cross-compatible and self-compatible, but there was pollinator limitation also. This study will provide important information for the preservation More >

  • Open Access

    ARTICLE

    Estimation of Genetic Divergence and Character Association Studies in Local and Exotic Diversity Panels of Soybean (Glycine max L.) Genotypes

    Syed Ali Zafar1,*, Muhammad Aslam2, Haroon Zaman Khan3, Sehrish Sarwar1, Rao Saad Rehman4, Mariam Hassan1, Ramala Masood Ahmad2, Rafaqat A. Gill5, Basharat Ali6, Ibrahim Al-Ashkar7, Abdullah Ibrahim7, Md Atikur Rahman8, Ayman El Sabagh9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1887-1906, 2023, DOI:10.32604/phyton.2023.027679 - 11 April 2023

    Abstract The availability of favorable genetic diversity is a thriving vitality for the success of a breeding program. It provides a firm basis of selecting superior breeding lines for the development of high yielding crop genotypes. In this context, present investigation aimed to generate information on genetic divergence and character association in a diversity panel of 123 local and exotic soybean genotypes. Analysis of variance revealed significant response of the evaluated genotypes based on studied attributes. It depicted the probability of selecting desirable soybean genotypes by focusing on character association studies and genetic diversity analysis. Correlation… More >

  • Open Access

    ARTICLE

    Introgression of Drought Tolerance into Elite Basmati Rice Variety through Marker-Assisted Backcrossing

    Muhammad Sabar1,2,#, Shahzad Amir Naveed1,3,4,#,*, Shahid Masood Shah5, Abdul Rehman Khan5, Muhammad Musaddiq Shah6, Tahir Awan1, Muhammad Ramzan Khan3, Zaheer Abbas3, Muhammad Arif1,7,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1421-1438, 2023, DOI:10.32604/phyton.2023.025801 - 09 March 2023

    Abstract Drought is one of the major abiotic threat to rice production in the context of climate change. Super Basmati is an elite, fine grain basmati rice variety grown in Punjab, Pakistan. Due to drought sensitive in nature, its yield has been facing an alarming situation in production because of gradual decrease in irrigated water for a couple of years. Three reported novel QTLs for drought tolerance were selected for incorporation into Super Basmati by employing marker assisted selection strategy. IR55419-04 with novel QTLs was used as a donor parent. Foreground selection was performed by applying… More >

  • Open Access

    ARTICLE

    Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters

    Jameel M. Al-Khayri1,*, Salha M. Alshamrani2, Adel A. Rezk1, Wael F. Shehata1, Mustafa I. Almaghasla3, Tarek A. Shalaby3,4, Ahmed M. Saad5, Fatmah A. Safhi6, Muhammad N. Sattar7, Arafat A. Abdel Latef8, Mahmoud Z. Sitohy5, Abdallah A. Hassanin9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1493-1512, 2023, DOI:10.32604/phyton.2023.027375 - 09 March 2023

    Abstract Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared among traits inside each group. A positive correlation was likewise noticed between the first and the second groups.… More >

  • Open Access

    ARTICLE

    Conventional Breeding and Molecular Markers for Blast Disease Resistance in Rice (Oryza sativa L.)

    Adel A. Rezk1,2, Mohamed M. El-Malky3, Hossam S. El-Beltagi1,4,*, Mohammed Al-daej1, Kotb A. Attia5,6

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 725-746, 2023, DOI:10.32604/phyton.2022.024645 - 29 November 2022

    Abstract Monogenic lines, which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method. The results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations (Sakha, Gemmeza, and Zarzoura in Egypt); Pia, Pik, Pik-p, Piz-t, Pita, Pi b, Pi, Pi 19 and Pi 20. While, the genes Pii, Pik-s, Pik-h, Pi z, Piz-5, Pi sh, Pi 3, Pi 1, Pi 5, Pi 7, Pi 9, Pi 12, Pikm and Pita-2 were highly resistant at the same locations. Clustering analysis confirmed the results, which divided into two groups; the first one included all the susceptible genes,… More >

  • Open Access

    REVIEW

    Research Progress and Application of Plant Branching

    Yaru Yang1, Yulan Hu1, Ping Li1, John T. Hancock2, Xiangyang Hu1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 679-689, 2023, DOI:10.32604/phyton.2023.024904 - 29 November 2022

    Abstract Plant branching development plays an important role in plant morphogenesis (aboveground plant type), the number and angle of branches are important agronomic characters that determine crop plant type. Effective branches determine the number of panicles or pods of crops and then control the yield of crops. With the rapid development of plant genomics and molecular genetics, great progress has been made in the study of branching development. In recent years, a series of important branching-related genes have been validated from Arabidopsis thaliana, rice, pea, tomato and maize mutants. It is reviewed that plant branching development is More >

  • Open Access

    ARTICLE

    Breeding Enrichment of Genetic Variation of Grain Yield and Its Attributes in Bread Wheat under Drought Stress and Well Irrigation

    Dina Swelam1, Abdel Hamid Salem2, Manal Hassan1, Mohammed Ali2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2699-2717, 2022, DOI:10.32604/phyton.2022.022651 - 29 August 2022

    Abstract Drought stress (DS) is one of the most critical environmental abiotic stresses for wheat production in the arid environments. Selection of high-yielding genotypes tolerant to DS can play a significant role in mitigation the negative impacts associated with DS. In the present study, generation means analysis (GMA) was used to study the performance of two crosses under well irrigation (WI) and deficit irrigation [cross I (Line 44 × Shandweel-1) and cross II (Line 20 × Sakha 93)]. Significant differences were observed for days to heading (DH), days to maturity (DM), plant height (PH), spike length (SL), number of spikes… More >

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