N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*
BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175
Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long… More >
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