Eliana Arias-Pérez1, Carlos Alberto Lecona-Guzmán1, Federico Antonio Gutiérrez-Miceli1, Joaquín Adolfo Montes-Molina1, Nancy Ruiz-Lau1,2,*
Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1741-1748, 2021, DOI:10.32604/phyton.2021.016004
- 28 June 2021
Abstract The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L−1 BA + 1 mg · L−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L−1 BA + 5.7 mg · L−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate More >
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