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    ARTICLE

    Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat. A freeze-fracture study

    JUAN CARLOS CAVICCHIA*, GUSTAVO GUEMBE, MABEL FÓSCOLO

    BIOCELL, Vol.34, No.2, pp. 81-90, 2010, DOI:10.32604/biocell.2010.34.081

    Abstract In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe ‘en face’ the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still… More >

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