Olga S. Tsiftsoglou^1,*, Vladimir Mihailovic², Nikola Sreckovic², Jelena S. Katanic Stankovic³, Kyriakos Michail Dimitriadis¹, Michalis K. Stefanakis⁴, Diamanto Lazari¹

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075566 - 30 January 2026

Abstract Achillea species are known for their healing properties since ancient times. There is extensive literature on their pharmacological action due to their bioactive compounds. The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst. and Kit., A. grandifolia Friv. and A. millefolium L. The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis, and the results were consistent with the spectrophotometrically determined total phenolic (TP: 125.42–191.98 mg/g) and total flavonoid (TF: 47.34–180.02 mg/g) contents. All the extracts were tested More >

Amine Batbat^1,2, Khaoula Habbadi², Mohamed Jeddi³, Samiah Hamad Al-Mijalli⁴, Hanae Naceiri Mrabti⁵, Fahad M. Alshabrmi⁶, Naif Hesham Moursi⁷, Hassane Greche¹, Naoufal El Hachlafi^8,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.072398 - 30 January 2026

Abstract Origanum elongatum (OE) is an aromatic, medicinal plant endemic to Morocco that is widely used in traditional medicine due to its biological properties. This study aimed to elucidate the chemical composition of the essential oil (EO) obtained from O. elongatum (OEEO) at three stages of its life cycle, including vegetative stage (OEEO-VS), flowering stage (OEEO-FS), and post-flowering (OEEO-PFS), as well as to evaluate its biological and antiradical characteristics. The chemical analysis of the essential oil was conducted using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was evaluated in vitro through distinct methodologies, namely, disc diffusion and microatmosphere assay;… More >

Yousra Hammouti^1,2,*, François Mesnard², Oussama Khibech³, Mohamed Taibi¹, Yousra Belbachir³, El Hassania Loukili⁴, Reda Bellaouchi⁵, Abdeslam Asehraou⁵, Mohamed Addi^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3563-3583, 2025, DOI:10.32604/phyton.2025.071139 - 01 December 2025

Abstract Centaurium erythraea Rafn (“Gosset El Haya”) has long been prized in North African folk medicine, yet Moroccan chemobiological data remain scarce. Ethanol extracts of northern Moroccan aerial parts were profiled by high-performance liquid chromatography (HPLC) and found rich in phenolics, dominated by 4-hydroxybenzoic acid (57.8%) and naringin (10.3%). The extract exhibited strong antioxidant power in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, with a half-maximal inhibitory concentration (IC₅₀) of ≈74 µg mL⁻¹, and a total antioxidant capacity (TAC) of ≈201 µg mL⁻¹ and selective antimicrobial activity, sharply inhibiting Aspergillus niger, Penicillium digitatum, and Rhodotorula glutinis while sparing Staphylococcus aureus. In-silico absorption, distribution, metabolism,… More >

Heba A. S. El-Nashar^1,*, Ahmed T. Negmeldin^2,3,*, Aziza El Baz⁴, Marizé Cuyler⁵, Brandon Alston⁵, Namrita Lall^5,6,7, Naglaa S. Ashmawy^1,8,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3269-3281, 2025, DOI:10.32604/phyton.2025.067998 - 29 October 2025

Abstract Pimenta dioica is a tropical Caribbean tree belonging to the family Myrtaceae, widely used in various human activities, including perfume production, food flavoring, natural pesticides, and medicine. This study aimed to explore the chemical composition of Pimenta dioica flower essential oil obtained via hydrodistillation using GC-MS analysis. Additionally, the oil’s tyrosinase inhibitory activity was investigated. The effectiveness of the oil’s major constituents in binding to tyrosinase was also evaluated through molecular docking simulations. GC-MS analysis identified fifteen compounds, with eugenol (70.59%) as the major component, followed by β-myrcene (10.54%), limonene (8.55%), β-ocimene (4.92%), α-phellandrene (1.39%), and linalool… More >

Mengting Huang, Meiting Lu, Li Yang, Jiwen Long, Li Zhou^*

Journal of Renewable Materials, Vol.13, No.10, pp. 2077-2090, 2025, DOI:10.32604/jrm.2025.02025-0122 - 22 October 2025

Abstract Peach gum polysaccharide (PGP), a readily available natural polysaccharide, boasts substantial potential across diverse applications, yet its practical utility is severely limited by its vulnerability to bacterial growth and limited antioxidant activity. Herein, we introduced a simple and effective method to enhance the antibacterial and antioxidant properties of PGP by conjugating it with salicylic acid (SA). Cytotoxicity evaluation results confirmed that the resulting PGP-SA retains the excellent biocompatibility of PGP. Notably, PGP-SA demonstrates outstanding antibacterial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria, outperforming non-modified PGP. Its antibacterial mechanism is hypothesized to stem from… More > Graphic Abstract

Longgang Wang^2,3,#, Xiangxun Zhuansun^2,3,#, Yao Li^2,3, Qili Yao^2,3, Qi Liu^2,3,*, Huijing Lin^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 441-454, 2025, DOI:10.32604/phyton.2025.061720 - 06 March 2025

Abstract Fructus Ligustri Lucidi (FLL) refers to the dried mature fruit of Ligustrum lucidum Ait., a species from the Oleaceae family, widely distributed across East Asia and India. This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil (FLLO) to develop an efficient and practical extraction method. Additionally, the chemical composition of FLLO was analyzed, and its antioxidant, antimicrobial, and cytotoxic activities were evaluated. FLLO was extracted using supercritical CO₂ extraction, and response surface methodology was applied to optimize the extraction parameters: pressure of 16 MPa, temperature of 40°C, and extraction time of 40… More > Graphic Abstract

Abida Aziz¹, Shafiqa Bano¹, Mubshar Hussain², Muhammad Farooq Azhar³, Ghulam Yasin³, Naila Hadayat⁴, Iqra Arooj⁵, Abeer Hashem⁶, Ajay Kumar⁷, Elsayed Fathi Abd_Allah⁸, Qamar uz Zaman^9,10,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1647-1665, 2024, DOI:10.32604/phyton.2024.050974 - 30 July 2024

Abstract Wheat (Triticum aestivum L.) exhibits a greater capacity for cadmium (Cd) absorption compared to other cereal crops, leading to elevated daily Cd intake, and posing a significant threat to public health. For the mitigation of Cd stress in sustainable and environmentally friendly way, a pot study was designed by using exogenous application of various biostimulants, i.e., Nigella sativa and Ocimum sanctum extracts: 0%, 10%, and 20% in combination with the chelating agent ethylenediaminetetraacetic acid (EDTA) using 0 and 5 mg kg under various levels of Cd stress (i.e., 0, 5, 10, and 15 mg kg soil). Results revealed… More > Graphic Abstract

Ayda Khadhri¹, Mohamed Mendili¹, Marwa Bannour-Scharinger¹, Eric Masson², Antonio Pizzi^2,*

Journal of Renewable Materials, Vol.12, No.4, pp. 827-842, 2024, DOI:10.32604/jrm.2024.046950 - 12 June 2024

Abstract This study is the first to evaluate the phytochemical content and biological properties of Tunisian T. platyphyllos Scop. A total of 23 compounds of essential oils were identified by gas chromatography-mass spectrometry (GC-MS) analysis of bracts and fruit extracts. The results show that oxygenated monoterpenes were the dominant class of essential oils. The phenolic composition was investigated by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The analysis showed that the chemical profiles of the ethanolic extracts of bracts and fruits are substantially similar. The highest polyphenol content was found in the ethanolic extracts of the fruits (7.65… More > Graphic Abstract

Jiaqi Lang¹, Mingyan Ye¹, Ya Luo¹, Yueheng Wang¹, Zhifen Shi^1,2, Xiaotian Kong^1,3, Xuan Li¹, Nong Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 789-806, 2024, DOI:10.32604/phyton.2024.049088 - 29 April 2024

Abstract This study aimed to examine the effects of inoculating Fritillaria taipaiensis P.Y.Li leaves with different strains of potassium-solubilizing bacteria (KSB), or combinations thereof, focusing on aspects of photosynthesis and physiological and biochemical characteristics. At present, some studies have only studied the rhizosphere microbial community characteristics of F. taipaiensis and have not discussed the effects of different microbial species on the growth promotion of F. taipaiensis. This paper will start from the perspective of potassium-solubilizing bacteria to conduct an in-depth study. Seed cultivation commenced at the base with three different KSBs in early October 2022. The growth of F. taipaiensis… More >

Ain Raal^1,*, Linda Rusalepp², Tatiana Chiru³, Nicolae Ciobanu³, Kelly Talvistu¹, Michelle Shusta¹, Oleh Koshovyi^1,4, Tõnu Püssa²

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2965-2979, 2023, DOI:10.32604/phyton.2023.042723 - 24 October 2023

Abstract The fruits of the Sea buckthorn (Hippophae rhamnoides L.) are a popular plant food and a valuable health product. Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue, which needs valorization. The aim of the study was to estimate (by HPLC-MS/MS) the qualitative and quantitative content of polyphenolic compounds in different parts of H. rhamnoides (HR), and to determine the level of antioxidant activity of leaves and fruits (by DPPH, ABTS methods and ferrozine test). Up to 19 compounds were identified in different parts of HR. The fruits are rich in flavonoids, including… More >