Di Pan*, Yuhua Song∗,†
Molecular & Cellular Biomechanics, Vol.8, No.3, pp. 233-252, 2011, DOI:10.3970/mcb.2011.008.233
Abstract Cytoskeletal restraints affect force-regulated integrin function in cell adhesion. However, the structural and molecular basis underlying the effect of cytoskeletal restraints on b1 integrin binding to fibronectin is still largely unknown. In this study, we used steered molecular dynamics simulations to investigate the changes in glycosylated b1 integrin-fibronectin binding and in conformation and structure of the glycosylated b1 I-like domain-FN-III9 - 10 complex caused by altered restraints applied to b1 I-like domain. The results revealed that imposition of the increased constraints on b1 integrin increased resistance to force-induced dissociation of the b1 I-like domain-fibronectin complex.… More >