Xiaoqing Jiang^1,2,#, Pengchong Li^1,2,#, Hongchuang Liu^1,2, Wenjie Dong^1,2, Wenjing Liu^1,2, Di Wu^1,2, Jianping Xue^1,2, Fenglan Zhao^1,2,*, Yongbo Duan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074434 - 30 January 2026

Abstract As its tuberous alkaloids induce valuable pharmacological effects, Pinellia ternata has considerable clinical value. However, its production currently fails to meet its demand. In vitro microtuber culture, combined with salicylic acid (SA) elicitation, may provide an effective alternative to traditional field production. In this study, an in vitro P. ternata microtuber induction system was developed and used to evaluate SA-induced elicitation of alkaloid accumulation. The quality of in vitro microtubers was assessed by total alkaloid measurement, a cytotoxicity assay, and transcriptomic analysis. With or without SA treatment, P. ternata microtuber induction was achieved within 60 d using petiole-derived explants, with… More >

Iván De-la-Cruz-Chacón, Christian Anabí Riley-Saldaña, Marisol Castro-Moreno, Claudia Azucena Durán-Ruiz, Alma Rosa González-Esquinca^*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3509-3526, 2025, DOI:10.32604/phyton.2025.072586 - 01 December 2025

Abstract The seedling phase represents an initial and critical stage for the establishment of individuals in the ecosystem. During this stage, specialized metabolites contribute to survival; however, studies analyzing the presence of these molecules and the reasons for their production and accumulation are still scarce. Annonaceae is a botanical family recognized for the chemodiversity of its secondary metabolites; nearly 1000 alkaloids have been reported in approximately 150 adult specimens. The aim of this study was to determine whether alkaloid biosynthesis in Annonaceae is expressed from early stages. For this purpose, Annona macroprophyllata, Annona muricata, Annona purpurea, and Annona reticulata seedlings,… More >

Chen Chen^1,2, Fengqin Liu¹, Jianan Li², Bo Wang², Yuanming Xiao², Tao Sun¹, Guoying Zhou^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 973-986, 2025, DOI:10.32604/phyton.2025.062421 - 31 March 2025

Abstract Anisodus tanguticus (Maxim.) Pascher, a medicinal plant in the Solanaceae family, is widely distributed across the Qinghai-Tibet Plateau. Its medicinal properties, particularly the tropane alkaloids, are influenced by various ecological factors, but the underlying ecological mechanisms remain poorly understood. This study aimed to investigate how key environmental variables influence both the morphological traits and tropane alkaloid contents of A. tanguticus, with the goal of providing data to support the sustainable cultivation and management of this species. We collected samples from 71 sites across its natural habitat, analyzing the effects of factors such as soil nutrients, altitude, and… More >

Liping Yang^1,#, Xin Wan^2,3,#, Runyang Zhou¹, Yingdan Yuan^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.1, pp. 81-96, 2024, DOI:10.32604/phyton.2023.045389 - 26 January 2024

Abstract As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemical properties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the gene family members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and the expression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families, including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains and motifs showed that the types, quantities, and orders of domains and motifs were similar among members of the More >

Kesheng Lin^1,2, Jiawen Zhou^1,2, Lijuan Han^1,2, Ning Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 285-296, 2023, DOI:10.32604/phyton.2022.023219 - 06 September 2022

Abstract As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline More >

Lohanathan Bharathi Priya^1,#, Balamuralikrishnan Balasubramanian^2,#, Balamurugan Shanmugaraj³, Shanmugam Subbiah⁴, Rouh-Mei Hu⁵, Chih-Yang Huang^6,7,8,9,*, Rathinasamy Baskaran^5,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1129-1140, 2022, DOI:10.32604/phyton.2022.017707 - 14 February 2022

Abstract For thousands of years, plant based herbal medicines have been utilized by millions of people all over the world. Plant materials or products are used in different folk/traditional medical systems, such as the Chinese, African and Indian medical systems, like Siddha, Ayurveda, Unani, and Homeopathy. Tinospora cordifolia (TC) is a medicinal plant belonging to the family Menispermaceae. It is a big deciduous, climbing shrub growing prevalently in the tropical part of Indian subcontinent regions such as India, Pakistan, Nepal, Bhutan, Bangladesh and Srilanka, and in Myanmar, and China. Guduchi, Giloy, Shindilkodi, and Amritha are all the… More >

JIANFEI GAO¹, KANGNING XIONG^2,*, WEIJIE LI¹, WEI ZHOU³

BIOCELL, Vol.46, No.1, pp. 285-296, 2022, DOI:10.32604/biocell.2021.016253 - 29 September 2021

Abstract Kadsura coccinea (Lem.) is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds. The non-editable part of the fruit, i.e., the seed and peel, represents more than 60% of the fruit and is considered a biowaste. This significantly restricts the development of the K. coccinea fruit industry. Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K. coccinea. Herein, we evaluated K. coccinea fruit peel, pulp, and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes. The… More >

Imad Ahmad^1,2, Haroon Khan^2,*, Muhammad Usman Amin¹, Shah Khalid³, Tapan Behl⁴, Najeeb Ur Rahman¹

Oncologie, Vol.23, No.3, pp. 321-333, 2021, DOI:10.32604/Oncologie.2021.018296 - 26 September 2021

Abstract Punarnavine (PN) has been isolated from the roots of Boerhaavia diffusa L. (Nyctaginaceae). It is an important medicinal plant with a vast historical ethnopharmacological background. Its identification resulted from the interrogation of ‘Punarnava’, a tribal medicinal product. The molecule exhibited its position as incipient anticancer therapeutic agent. The inhibition of NFκB, ATF-2, c-Fos, and CREB-1 are one of the underlying mechanisms of anticancer action along with modification of the immune system. These signalling molecules are upregulated in the cancer microenvironment. Punarnavine also modified the release of interleukins, i.e., upregulated IL-2, IL-7, IL-10, IL-12, IFN-γ, while downregulated… More >

Mohammed Ali Ashehri^*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1749-1762, 2021, DOI:10.32604/phyton.2021.016290 - 28 June 2021

Abstract In most of Arabian courtiers Rhazya stricta is extensively utilized in public medicine for several diseases treatment. In this study, crude alkaloid extract of R. stricta (CAERS) coated with silver nanoparticles (CAERS-AgNPs) as potential treatment against diabetes in DM animal model was evaluated. Swiss albino male rats (n = 80) were injected with STZ to induce Diabetes Mellitus type-2 (DM). DM-rats were injected different doses of CAERS or CAERS-nanoparticles (CAERS-NPs) for 2 months. The results exhibited that mRNA expression of insulin and insulin receptor was down-regulated, activity levels of antioxidant enzymes were decreased, generation of ROS mediated DNA adducts… More >

Guiming Deng^1,2,3, Ou Sheng^1,2,3, Fangcheng Bi^1,2,3, Chunyu Li^1,2,3, Tongxin Dou^1,2,3, Tao Dong^1,2,3, Qiaosong Yang^1,2,3, Huijun Gao^1,2,3, Jing Liu⁴, Xiaohong Zhong⁴, Miao Peng⁴, Ganjun Yi^1,2,3, Weidi He^1,2,3, Chunhua Hu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1101-1130, 2020, DOI:10.32604/phyton.2020.010970 - 09 November 2020

Abstract Banana (Musa spp.) is an ancient and popular fruit plant with highly nutritious fruit. The pseudo-stem of banana represents on average 75% of the total dry mass but its valorization as a nutritional and industrial by-product is limited. Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts. This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem. Hereby, we identified and quantified 373 metabolites from a diverse range of classes including, alkaloids, flavonoids, lipids, phenolic acids,… More >