MANOOCHEHR MESSRIPOUR1, AZADEH MESRIPOUR2
BIOCELL, Vol.37, No.2, pp. 17-21, 2013, DOI:10.32604/biocell.2013.37.017
Abstract Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age,
this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal
synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal
synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated
in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during
25 min was measured by HPLC, employing electrochemical… More >
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