Sushuang Liu1,2,#, Yanmin Liu1,2,#, Chundong Liu1, Xingwang Yu2,3, Hao Ma2,4,*
Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 577-590, 2023, DOI:10.32604/phyton.2022.023738
- 12 October 2022
Abstract Drought is a major abiotic stress limiting agricultural crops production worldwide. In our study, we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea. ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain. Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein. In the yeast assay system, the full-length of ZF2 did not show transcriptional activation. Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones. The promoter region of ZF2 contained multiple stress- and hormone-related cis-elements. Overexpression of More >