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  • Open Access

    ARTICLE

    Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

    Yulin Luo1,2,#, Panpan Yang2,#, Mengmeng Bi2, Leifeng Xu2, Fang Du3,*, Jun Ming2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 1057-1066, 2024, DOI:10.32604/phyton.2024.051197 - 28 May 2024

    Abstract The xylem undergoes physiological changes in response to various environmental conditions during the process of plant growth. To understand these physiological changes, it is extremely important to observe the transport of xylem. In this study, the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin section. Methods for introducing a fluorescent tracer into the xylem of the stems were evaluated. Then, the transport rule of 5(6)-Carboxyfluorescein diacetate (CFDA) in the xylem of the stem of L. lancifolium was studied by fluorescence dye in live cells tracer technology. The results showed… More >

  • Open Access

    ARTICLE

    A Simple Clear Technique in Observing Vascular Development of Grape Ovary

    Teng Fei1, Youmei Li1, Bo Li2, Zhaosen Xie1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2117-2132, 2023, DOI:10.32604/phyton.2023.028208 - 29 May 2023

    Abstract The vascular system of the grapevine (Vitis vinifera L.) flower is a channel for transporting water and nutrients to the ovary. It plays an important role in the development of the ovary and fertilization through pollination. However, the vascular bundles in the flower are so tiny that they are difficult to sample and observe by traditional slicing techniques. In this study, ‘Summer Black’ grape flowers were selected as the test materials, and the tissue samples were treated by the optical clearing technique. After simple compaction, the structure and development of the vasculature were observed by common… More >

  • Open Access

    ARTICLE

    Analysis of Water Transport inside a Plant Xylem Vessel with Pitted Thickening

    Tianyu Xu, Lixiang Zhang*

    FDMP-Fluid Dynamics & Materials Processing, Vol.16, No.3, pp. 525-536, 2020, DOI:10.32604/fdmp.2020.09618 - 25 May 2020

    Abstract In this article, computational fluid dynamics (CFD) are used to explore the dynamics of water transport inside the pitted thickening of a plant xylem vessel. A pitted thickening model combined with the Bernoulli equation is used to analyze the influence of various factors (namely, the inner diameter, thickening width, thickening height, thickening spacing, number of laps and adjacent pit axial rotation). The pressure drop and the flow resistance coefficient are the variable parameters for our analysis. The results show that these two parameters are proportional to the thickening height and thickening width, and inversely proportional More >

  • Open Access

    ARTICLE

    Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum

    Maryam Monazzah1, Sattar Tahmasebi Enferadi1,*, Zohre Rabiei1 and Alessandro Mattiello2

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 149-159, 2019, DOI:10.32604/phyton.2019.05053

    Abstract Helianthus annuus L. as an oil seed crop is widely grown throughout the world. One of the most destructive diseases of sunflower is stem rot caused by Sclerotinia sclerotiorum. Oxalic acid is the major virulence factor of this necrotrophic pathogen. It is important to further investigate plant responses to this non-specific toxin. Therefore, in the present study, we compared the patterns of total soluble proteins and xylem morphology of partially resistant and susceptible sunflower lines after treatment with Sclerotinia culture filtrate. The basal stems of both lines were treated with 40 mM oxalic acid (pH… More >

  • Open Access

    ARTICLE

    Morphological differences of vessels in the secondary xylem of columnar and standard apple trees

    Zhang YG, SX Guo, HY Dai

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 229-232, 2012, DOI:10.32604/phyton.2012.81.229

    Abstract The length, dimension and type of the vessel elements in the secondary xylem of columnar and standard apple trees were studied by isolation method and micrograph. Results showed that the shoot xylem vessel cells were reticulate and pitted in columnar and standard apples. Most of the end walls were simple perforation plates. Compared with columnar apple trees, standard apple trees showed more abnormal cells in the xylem vessel cells. The average diameter of the xylem vessel cells of shoots was significantly wider in columnar apple (43.27μm) than in standard apple, (32.64 μm). Length of the More >

  • Open Access

    ARTICLE

    Leaf and stem anatomy and micromorphology in two cut flower cultivars of Rosa hybrida L.

    Cohen1 G, L Mascarini1, CC Xifreda2

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 199-204, 2012, DOI:10.32604/phyton.2012.81.199

    Abstract Micromorphological and anatomical differences in leaf epidermis and stem xylem of Rosa hybrida ‘Lovely Red ‘ (LR) and ‘Rouge Baiser’ (RB), commercially grown in Buenos Aires, Argentina, were examined in the lower half of the terminal leaflet of 5-foliate leaves and in the basal part of stem, respectively. Digital images obtained from SEM and a phase contrast microscope were used with this purpose. Epicuticular wax, which was present on both sides of the leaves, was more abundant in LR than in RB. Stomata were only present on the abaxial side; they were 111 and 129 per… More >

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