Jianbo Fei^1,2,4, Zhibo Liu¹, Piwu Wang^1,3, Jing Qu², Siyan Liu¹, Shuyan Guan^1,3,*, Yiyong Ma^1,3

Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3617-3635, 2024, DOI:10.32604/phyton.2024.052704 - 31 December 2024

Abstract The WRKY transcription factors play important roles in various biological processes such as plant development, defense regulation, and stress response. Despite this, there is limited information available on drought-related WRKY genes in maize. Through RNA sequencing (RNA-seq) analysis, a WRKY transcription factor called ZmWRKY25 was identified in this study. ZmWRKY25 belongs to Group II and is localized in the nucleus. Protein interaction analysis revealed that ZmWRKY25 interacts with several proteins involved in the abscisic acid (ABA) signal pathway. Expression of ZmWRKY25 was found to be up-regulated in response to drought, salt, and ABA treatments in tobacco plants. More >

Enyan Chen^1,#, Jingyu Jia^1,3,#, Jiaju Sun¹, Jie Wang², Xinxin Chen¹, Xin Li^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2837-2856, 2024, DOI:10.32604/phyton.2024.057932 - 30 November 2024

Abstract During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase flavonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced flavonoid biosynthesis in C. sativus, we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy-five significantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22… More >

Dandan Yuan, Ju Cai, Tao Zhang, Sisi Wang, Xiuliu Yang, Yan Li^*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1875-1896, 2024, DOI:10.32604/phyton.2024.052190 - 30 August 2024

Abstract The WRKY transcription factor gene family is one of the unique gene families in plants. It plays an important role in response to abiotic stresses such as cold and drought, hormone signal transduction, regulation of biosynthesis, leaf senescence seed germination, etc. However, little information is available about WRKY transcription factors in Verbena bonariensis. In this study, 70 VbWRKY genes were identified from the whole genome. The phylogenetic analysis of the WRKY gene family in V. bonariensis and Arabidopsis shows that the WRKY genes in V. bonariensis can be divided into three groups: I, II, and III, which contain 13, 47, and… More >

Mingchuan Yang¹, Jia Zheng², Wenhui Yu¹, Yanghua Li², Yali Wang¹, Zilu Zhang¹, Zhenhui Kang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1503-1521, 2024, DOI:10.32604/phyton.2024.051718 - 30 July 2024

Abstract Sorghum is not only an important bio-energy crop but also a vital raw material for brewing. Exogenous copper affects the growth and metabolism of crops in specific ways. This study identified 8475 differentially expressed genes (DEGs) by high-throughput transcriptome sequencing in the sorghum cultivar ‘Jinnuoliang 2’ after 24 h of treatment with 10 mM CuSO. Using GO analysis, 476 genes were functionally annotated, which were mainly related to catabolism and biosynthetic processes. Additionally, 90 pathways were annotated by employing the KEGG analysis. Among them, glutathione metabolism and peroxisome were induced, while photosynthesis, photosynthesis-antenna protein, and… More >

Yueying Liu^#, Yuqing Wang^#, Lijiao Gu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2937-2946, 2023, DOI:10.32604/phyton.2023.043306 - 24 October 2023

Abstract WRKY transcription factors play important roles in plant growth, development, and stress responses. Our previous research has shown that the GhWRKY91 gene can delay age-, abscisic acid (ABA)-, and drought-induced leaf senescence when overexpressed in transgenic Arabidopsis plants. To explore in more depth the biological functions of the GhWRKY91 gene, we further observed the root growth of overexpressing transgenic Arabidopsis thaliana under ABA and drought treatment. In this study, we transplanted the germinated seeds of wild-type (WT) and three transgenic lines (OE-12, OE-13 and OE-20) to 1/2 MS solid medium containing ABA and different concentrations of mannitol (simulated… More >

Huaqiao Ding^*, Lihui Mao, Qingcheng Zou, Wei Hu, Xuerui Cao, Qing Dong^*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 773-789, 2022, DOI:10.32604/phyton.2022.018075 - 09 December 2021

Abstract Snapdragon (Antirrhinum majus) is one of the most widely cultivated grass flowers in the world. WRKY transcription factors, VQ proteins and their interactions play crucial roles in plant response to abiotic stresses. However, little is known about WRKY and VQ gene families in snapdragon. In the present study, WRKY and VQ genes and their interactions were comprehensively analyzed in snapdragon using bioinformatics approaches, and their expression in response to drought and cold stresses was examined using real-time PCR. A total of 67 AmWRKY genes were identified in snapdragon, which were classified into different groups or subgroups based on phylogenetic… More >

QICHAO WANG¹, WUJING ZENG¹, BASHARAT ALI², XUEMIN ZHANG³, LING XU^1,*, ZONGSUO LIANG^1,*

BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282 - 22 April 2021

Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From the structural… More >