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  • Open Access

    ARTICLE

    Transcriptome analysis combined with metabolome analysis reveals the significant functions of CesA genes in cotton (Gossypium hirsutum) fiber length development

    ZHENKUI CUI1,#,*, GUIQIN SUN2,#,*, QUANZHI ZHAO1

    BIOCELL, Vol.46, No.9, pp. 2133-2144, 2022, DOI:10.32604/biocell.2022.020512

    Abstract Cotton is widely distributed worldwide, and improving the quality of its fiber is one of the most important tasks in cotton breeding. Cotton fibers are primarily composed of cellulose, which is synthesized by CesA complexes (CSCs). However, the functions of CesA genes in cotton fiber development have not been comprehensively analysed. In this study, the cotton transcriptome and metabolome were used to investigate the function of CesA genes in fiber development. Finally, 321 metabolites were obtained, 84 of which were associated with the corresponding genes. Interestingly, a target gene named Gh_A08G144300, one of the CesA gene family members, was closely… More >

  • Open Access

    ARTICLE

    Application of VIGS system to explore the function of common wheat TaADF7

    HUIZE CHEN1,2,*, XINGTIAN JIA1,2, HAIYAN RAN1,2, JINGRONG NIU1,2, MEITING DU1

    BIOCELL, Vol.46, No.2, pp. 559-565, 2022, DOI:10.32604/biocell.2022.017437

    Abstract As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its gene function. Virus-induced gene silencing (VIGS) is an important tool for the rapid analysis of plant gene function. In this study, the gene silencing system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used to silence the wheat phytoene desaturase (TaPDS) and actin depolymerization factor (TaADF7) genes and determine the effect of gene silencing on wheat. TaPDS was used as an indicator gene to determine the feasibility of VIGS system, while TaADF7 was used as a test gene to determine its… More >

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