BEEMNET MENGESHA KASSAHUN1,#, BEUM-CHANG KANG2,#, SU-JI BAE2, YE JIN NAM1, GRETEL FONSECA MUNDO1, GA-HUI KANG1, KYOUNGOOK KIM3, JEUNG-SUL HAN1,4,*
BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353
- 26 January 2021
Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome
sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar
has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are
in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of
an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly
process together with single-guide RNA (sgRNA) in… More >
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