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  • Open Access

    ARTICLE

    Ganoderic acid A ameliorates renal fibrosis by suppressing the expression of NPC1L1

    TIANYUN HAN#, ZHONG LI#, LUONING ZHANG, LINSHEN XIE*

    BIOCELL, Vol.48, No.11, pp. 1625-1638, 2024, DOI:10.32604/biocell.2024.055029 - 07 November 2024

    Abstract Objective: The study aimed to explore the protective mechanism of Ganoderic acid A (GAA) in renal fibrosis and to verify that GAA can ameliorate renal fibrosis by regulating the Niemann-pick C1-like 1 (NPC1L1) gene. Methods: Transforming growth factor beta1 (TGF-β1) was used to treat Human Kidney-2 (HK-2) cells to establish a renal fibrosis model. The differentially expressed genes in the control (CTRL) group, TGF-β1 group, and TGF-β1 + GAA group were screened via transcriptome sequencing technology and verified by qPCR and Western blot experiments. The NPC1L1 gene overexpression plasmid was constructed. The expression levels of N-cad, E-cad,… More >

  • Open Access

    ARTICLE

    Integrated Transcriptomics and Metabolomics Analysis for the Mechanism Underlying White-to-Pink Petal Color Transition in Hibiscus mutabilis Flowers

    Xiaodong Shi*, Tingyu Wang, Sui Ai, Jiasi Li

    Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2571-2581, 2024, DOI:10.32604/phyton.2024.056606 - 30 October 2024

    Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract

    Integrated Transcriptomics and Metabolomics Analysis for the Mechanism Underlying White-to-Pink Petal Color Transition in <i>Hibiscus mutabilis</i> Flowers

  • Open Access

    ARTICLE

    Single-cell transcriptomics reveals T-cell heterogeneity and immunomodulatory role of CD4+ T native cells in Candida albicans infection

    KERAN JIA1, YANHAO ZHANG1, MENGYU JIANG2, MENGGE CUI2, JIA WANG2, JIAJIA ZHANG2, HUIHAI ZHAO2, MENGYAN LI2, HUA WANG2, QUANMING ZOU1,#,*, HAO ZENG1,#,*

    BIOCELL, Vol.48, No.9, pp. 1355-1368, 2024, DOI:10.32604/biocell.2024.051383 - 04 September 2024

    Abstract Objective: Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms, including coordinated innate and adaptive immune responses, to neutralize invading fungi effectively. Exploring the immune microenvironment has the potential to inform the development of therapeutic strategies for fungal infections. Methods: The study analyzed individual immune cell profiles in peripheral blood mononuclear cells from Candida albicans-infected mice and healthy control mice using single-cell transcriptomics, fluorescence quantitative PCR, and Western blotting. We investigated intergroup differences in the dynamics of immune cell subpopulation infiltration, pathway enrichment, and differentiation during Candida albicans infection. Results: Our findings indicate that infiltration… More >

  • Open Access

    ARTICLE

    EuSHT Acts as a Hub Gene Involved in the Biosynthesis of 6-Hydroxyluteolin and Quercetin Induced by Salt Stress in Eucommia ulmoides

    Fuxin Li1, Enyan Chen1, Xinxin Chen1, Jingyu Jia1, Hemin Wang1, Jie Zhang1, Jianrui Sun1, Xin Li1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 2095-2113, 2024, DOI:10.32604/phyton.2024.054231 - 30 August 2024

    Abstract Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites. Flavonoids are natural compounds that possess many important biological activities, playing a significant role in the medicinal activity of Eucommia ulmoides (E. ulmoides). To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in E. ulmoides, a comprehensive analysis of metabolomics and transcriptomics was conducted. The results indicated that salt stress led to the wilting and darkening of E. ulmoides leaves, accompanied by a decrease in chlorophyll levels, and significantly induced malondialdehyde (MDA) and relative electrical conductivity. During salt stress, most metabolites in… More >

  • Open Access

    ARTICLE

    4-Hydroxy-2-Oxoglutaric Acid, A Key Metabolite Involved in Trypsin-Regulation of Arginine Metabolism in Hylocereus undatus during Storage

    Bairu Li1, Jingyu Jia1, Hemin Wang1, Jiaju Sun1, Enyan Chen1, Xin Li1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 885-900, 2024, DOI:10.32604/phyton.2024.050450 - 28 May 2024

    Abstract Trypsin, a novel superoxide scavenger, significantly enhances the storage quality of Hylocereus undatus (H. undatus). To elucidate the preservation mechanism of trypsin on H. undatus, a widely targeted metabolomic analysis, and transcriptomics analysis were conducted. Firstly, a total of 453 metabolites were identified, with organic acids and their derivatives constituting the largest proportion (25%). Amino acids and their metabolites, prominent among organic acids, were further analyzed. Among them, 73 metabolites were associated with amino acids, and 37 exhibited significant differences. The most enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was arginine biosynthesis (map00220), with polyamine metabolites… More >

  • Open Access

    REVIEW

    New perspectives on biology, disease progression, and therapy response of head and neck cancer gained from single cell RNA sequencing and spatial transcriptomics

    GERWIN HELLER1,*, THORSTEN FUEREDER1, ALEXANDER MICHAEL GRANDITS1, ROTRAUD WIESER1,2,*

    Oncology Research, Vol.32, No.1, pp. 1-17, 2024, DOI:10.32604/or.2023.044774 - 15 November 2023

    Abstract Head and neck squamous cell carcinoma (HNSCC) is one of the most frequent cancers worldwide. The main risk factors are consumption of tobacco products and alcohol, as well as infection with human papilloma virus. Approved therapeutic options comprise surgery, radiation, chemotherapy, targeted therapy through epidermal growth factor receptor inhibition, and immunotherapy, but outcome has remained unsatisfactory due to recurrence rates of ~50% and the frequent occurrence of second primaries. The availability of the human genome sequence at the beginning of the millennium heralded the omics era, in which rapid technological progress has advanced our knowledge… More >

  • Open Access

    ARTICLE

    Combining Transcriptomics and Metabolomics to Uncover the Effects of High-Energy Lithium-Ion Beam Irradiation on Capsicum annuum L.

    Libo Xie1, Xue Wang1, Luxiang Liu2,*, Chunmei Xu1, Yongdun Xie2, Hongchun Xiong2, Xinchun Han3, Mu Guo1

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2947-2964, 2023, DOI:10.32604/phyton.2023.042919 - 24 October 2023

    Abstract Hot pepper (Capsicum annuum L.) is consumed as one of the oldest domesticated crops all over the world. Although mutation breeding using radiation has been performed in hot peppers, little is known about the comparative analysis of mutagenic effects at the molecular level by ion beam irradiation. To comprehend the response mechanism of hot pepper to the ion beam, we used a mutant with favorable economic characteristics induced by lithium-ion beam irradiation to investigate the biological effects. The results indicated that the lithium-ion beam had a positive effect on important agronomic traits, particularly yield unit, but… More >

  • Open Access

    REVIEW

    Progress in research on tumor microenvironment-based spatial omics technologies

    FANGMEI XIE1,#, NAITE XI1,#, ZEPING HAN1,#, WENFENG LUO1, JIAN SHEN1, JINGGENG LUO2, XINGKUI TANG2, TING PANG1, YUBING LV1, JIABING LIANG1, LIYIN LIAO1, HAOYU ZHANG1, YONG JIANG1, YUGUANG LI3,*, JINHUA HE1,*

    Oncology Research, Vol.31, No.6, pp. 877-885, 2023, DOI:10.32604/or.2023.029494 - 15 September 2023

    Abstract Spatial omics technology integrates the concept of space into omics research and retains the spatial information of tissues or organs while obtaining molecular information. It is characterized by the ability to visualize changes in molecular information and yields intuitive and vivid visual results. Spatial omics technologies include spatial transcriptomics, spatial proteomics, spatial metabolomics, and other technologies, the most widely used of which are spatial transcriptomics and spatial proteomics. The tumor microenvironment refers to the surrounding microenvironment in which tumor cells exist, including the surrounding blood vessels, immune cells, fibroblasts, bone marrow-derived inflammatory cells, various signaling More >

  • Open Access

    ARTICLE

    Growth and Transcriptomics Analysis of Michelia macclurei Dandy Plantlets with Different LED Quality Treatments

    Zhaoli Chen, Ying Liu*, Bingshan Zeng, Qingbin Jiang, Shengkun Wang, Xiangyang Li

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2891-2906, 2023, DOI:10.32604/phyton.2023.030664 - 15 September 2023

    Abstract Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China, owing to its economic and practical importance. Light quality influences plantlet growth and development during tissue culture. However, the growth characteristic and molecular regulation of M. macclurei under different light quality conditions are not well understood yet. In this study, we investigated the morphological, chlorophyll content, and transcriptomic responses of M. macclurei plantlets under different light-emitting diode (LED) qualities, including white, blue, and red light. The results showed that blue light significantly increased plant height (21.29%) and leaf… More >

  • Open Access

    ARTICLE

    Transcriptomic and Physiological Analyses of Pigment Accumulation in Eucommia ulmoides ‘Hongye’

    Mengjiao Chen1,2, Jinhui Zhai3, Jiajia Zhang1,4,5,6, Hui Li1,2, Xinjiang Niu1,2, Yaxin Liu1,2, Yue Ren1,2, Hongyan Du1,4,5,6, Jingle Zhu1,4,5,6,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1027-1044, 2022, DOI:10.32604/phyton.2022.019106 - 24 January 2022

    Abstract Eucommia ulmoides ‘Hongye’ is a new ornamental variety of E. ulmoides with excellent red or purple foliage. We found that E. ulmoides ‘Hongye’ exhibited a gradual change from green to red colour under light conditions. However, the colouring mechanism in the leaves of E. ulmoides ‘Hongye’ remains unclear. In this study, we compared the pigment content and leaf colour index of E. ulmoides ‘Hongye’ at five stages with those of E. ulmoides ‘Xiaoye’, which was used as the control variety. The transcriptome sequencing data of the first-period (H1, green) and fifth-period (H5, red) leaves were also analysed and compared. The corresponding… More >

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