Jing Zhang^1,#, Huifen Qiao^1,#, Shenglong Wang^1,#, Jiawei Yuan¹, Qingsong Ba¹, Gensheng Zhang^1,2,*, Guiping Li^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2701-2718, 2025, DOI:10.32604/phyton.2025.070017 - 30 September 2025

Abstract Wheat stripe rust, a devastating disease caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), poses a significant threat to global wheat production. Growing resistant cultivars is a crucial strategy for wheat stripe rust management. However, the underlying molecular mechanisms of wheat resistance to Pst remain incompletely understood. To unravel these mechanisms, we employed high-throughput RNA sequencing (RNA-Seq) to analyze the transcriptome of the resistant wheat cultivar Mianmai 46 (MM46) at different time points (24, 48, and 96 h) post-inoculation with the Pst race CYR33. The analysis revealed that Pst infection significantly altered the expression of genes… More >

Siyang Ou¹, Liuyan Yang¹, Tingting Yuan¹, Mutong Li¹, Guohui Liao², Wanping Zhang¹, Guangdong Geng^1,*, Suqin Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2441-2460, 2025, DOI:10.32604/phyton.2025.068368 - 29 August 2025

Abstract Chive (Allium ascalonicum L.), a seeding-vernalization-type vegetable, is prone to bolting. To explore the physiological and molecular mechanisms of its bolting, bolting-prone (‘BA’) and bolting-resistant (‘WA’) chives were sampled at the vegetative growth, floral bud differentiation, and bud emergence stages. No bolting was observed in bolting-resistant ‘WA’ on the 130th day after planting, whereas the bolting reached 39.22% in bolting-prone ‘BA’, which was significantly higher than that of ‘WA’. The contents of gibberellins, abscisic acid, and zeatin riboside after floral bud differentiation in ‘WA’ were significantly less than in ‘BA’, whereas the indoleacetic acid content in… More >

Xiaoqin Li, Yongfu Zhang^*, Zhen Ren, Jiao Chen, Zuqin Qiao, Xingmei Tao, Xuan Yi, Kai Wang, Zhao Liu

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2529-2550, 2025, DOI:10.32604/phyton.2025.066894 - 29 August 2025

Abstract Soil salinization is a major abiotic stress that hampers plant development and significantly reduces agricultural productivity, posing a serious challenge to global food security. Akebia trifoliata (Thunb.) Koidz, a species within the genus Akebia Decne., is valued for its use in food, traditional medicine, oil production, and as an ornamental plant. Curcumin, widely recognized for its pharmacological properties including anti-cancer, anti-neuroinflammatory, and anti-fibrotic effects, has recently drawn interest for its potential roles in plant stress responses. However, its impact on plant tolerance to saline-alkali stress remains poorly understood. In this study, the effects of curcumin on… More >

Liangju Peng^1,2, Tingting Cai^1,2, Peihang Xu^1,2, Cong Chen³, Qingzhi Xiang^1,2, Yiping Zhu^1,2, Dingwei Ye^1,2,*, Yijun Shen^1,2,*

Oncology Research, Vol.33, No.9, pp. 2463-2489, 2025, DOI:10.32604/or.2025.064331 - 28 August 2025

Abstract Background: Studies have reported the special value of PANoptosis in cancer, but there is no study on the prognostic and therapeutic effects of PANoptosis in bladder cancer (BLCA). This study aimed to explore the role of PANoptosis in BLCA heterogeneity and its impact on clinical outcomes and immunotherapy response while establishing a robust prognostic model based on PANoptosis-related features. Methods: Gene expression profiles and clinical data were collected from public databases. Spatial heterogeneity of cell death pathways in BLCA was evaluated. Consensus clustering was performed based on identified PANoptosis genes. Cell death pathway scores, molecular, and… More >

Weiliang Qi^1,2,3,4,*, Wancang Sun⁵, Li Ma⁵, Xiaolong Li¹, Haiqing Liu¹, Cairong Yang⁶, Ziyao Wei¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 739-762, 2025, DOI:10.32604/phyton.2025.060712 - 31 March 2025

Abstract Brassica napus L. (B. napus), recognized as a significant cash and oil crop, faces challenges in popularization and application in northern China due to its limited cold resistance. Clarifying the mechanism of cold stress on gene regulation and signal transduction in B. napus is crucial. To address these issues, we conducted transcriptome sequencing and gene expression analysis, along with gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway profiling under natural (25°C) and cold (4°C) conditions in cold tolerant 16VHNTS309 and weak cold-resistant Tianyou 2238 B. napus seedlings. Enhanced genomic annotation was achieved through additional sequencing.… More >

Weimin Zhao¹, Guomin Shi^2,3, Jialei Guo⁴, Guifang He¹, Peilan Li¹, Xiaoying Ren¹, Leqi Yang¹, Taikun Qi¹, Tao He^1,5,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 393-406, 2025, DOI:10.32604/phyton.2025.059531 - 06 March 2025

Abstract Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils, as well as sexual reproduction via seeds. The phenomenon of vegetative organ vivipary is a complex biological process regulated by a network of genes. However, the developmental mechanism regulating bulbil vivipary in B. vivipara remains largely unexplored. This study investigated different developmental stages of B. vivipara using RNA sequencing and transcriptome analysis. Approximately 438 million high-quality reads were generated, with over 61.65% of the data mapped to the de novo transcriptome sequence. A total of 154,813 reads were matched in at least one More >

Yanlin Pan¹, Yibin Zhang¹, Xingui Wang¹, Hongbo Qin¹, Lunfa Guo^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 123-136, 2025, DOI:10.32604/phyton.2025.059598 - 24 January 2025

Abstract Derris fordii and Derris elliptica belong to the Derris genus of the Fabaceae family, distinguished by their high isoflavonoid content, particularly rotenoids, which hold significance in pharmaceuticals and agriculture. Rotenone, as a prominent rotenoid, has a longstanding history of use in pesticides, veterinary applications, medicine, and medical research. The accumulation of rotenoids within Derris plants adheres to species-specific and tissue-specific patterns and is also influenced by environmental factors. Current research predominantly addresses extraction techniques, pharmacological applications, and pesticide formulations, whereas investigations into the biosynthesis pathway and regulatory mechanism of rotenoids remain relatively scarce. In this study, we… More >

RYUL KIM^1,#, JOO KYUNG PARK^2,#, MINSUK KWON³, MINAE AN⁴, JUNG YONG HONG¹, JOON OH PARK¹, SUNG HEE LIM^1,*, SEUNG TAE KIM^1,*

Oncology Research, Vol.33, No.1, pp. 57-65, 2025, DOI:10.32604/or.2024.049054 - 20 December 2024

Abstract Background: Immune checkpoint inhibitors (ICIs) are effective in a subset of patients with metastatic solid tumors. However, the patients who would benefit most from ICIs in biliary tract cancer (BTC) are still controversial. Materials and methods: We molecularly characterized tissues and blood from 32 patients with metastatic BTC treated with the ICI pembrolizumab as second-line therapy. Results: All patients had microsatellite stable (MSS) type tumors. Three of the 32 patients achieved partial response (PR), with an objective response rate (ORR) of 9.4% (95% confidence interval [CI], 2.0–25.2) and nine showed stable disease (SD), exhibiting a disease… More >

Yuan Wang¹, Tianle Zhang¹, Xinfeng Zeng¹, Jiale Liu¹, Siyu Li¹, Siyu Yang¹, Shengnan Zhao¹, Abdullah Shah², Muhammad Saif Ullah², Guohui Qi^1,*, Peng Jia^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3331-3346, 2024, DOI:10.32604/phyton.2024.059402 - 31 December 2024

Abstract LONELY GUY (LOG) is a pivotal cytokinin-activating enzyme that plays an important role in plant growth, development, and stress responses. Walnut (Juglans regia), an important woody oilseed species, has not yet undergone systematic identification of its LOG gene family. In this study, we identified 17 JrLOG genes in the walnut genome, which are unevenly distributed across 11 chromosomes. JrLOG gene expansion was primarily driven by gene duplication, along with purifying selection. Members of the JrLOG family were categorized into five groups, each exhibiting analogous gene structures, featured motifs, and conserved domains. Transcriptome and quantitative real-time PCR analyses More >

Xiaodong Shi^*, Tingyu Wang, Sui Ai, Jiasi Li

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2571-2581, 2024, DOI:10.32604/phyton.2024.056606 - 30 October 2024

Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract