FABIANA ROCHA ARAÚJO1, BRUNO MACHADO BERTASSOLI2, ISABELLA CRISTINA SOUZA FÉLIX1, DOUGLAS MARINHO ABREU1, NATáLIA MELO OCARINO1, AMANDA MARIA SENA REIS3, JUNEO FREITAS SILVA4, ROGéRIA SERAKIDES1,*
BIOCELL, Vol.47, No.2, pp. 423-430, 2023, DOI:10.32604/biocell.2023.023689
- 18 November 2022
Abstract To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal hyperthyroidism. Twelve adult Wistar rats were divided into two groups, one control and one treated with daily administration of L-thyroxine by an orogastric tube (50 µg/animal/day) during pregnancy. Three days after delivery and confirmation of the mothers’ hyperthyroidism, the offspring were euthanized for the extraction of osteoblasts from the calvaria. At 7, 14, and 21 days, proliferation activity was assessed using MTT assay, while alkaline phosphatase (ALP) activity was assessed by the BCIP/NBT method. At… More >
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