MIAO YAN¹, DONGXUAN CHI², WEN WANG³, PEI PEI⁴, MIN XIE¹, SHUANGLING LI^1,*

BIOCELL, Vol.49, No.5, pp. 893-906, 2025, DOI:10.32604/biocell.2025.063622 - 27 May 2025

Abstract Background: Transcription factor Krüppel-like factor 3 (KLF3) may be involved in regulating inflammation and lymphocyte function. Immune dysfunction in sepsis involves both hyper-inflammation and immunosuppression. However, studies on T-lymphocyte KLF3 expression in sepsis are lacking. Methods: We induced sepsis in mice via cecal ligation and puncture (CLP), and their survival rate over 7 days was evaluated. To identify the immune status of these mice, we assessed their cytokine levels, organ damage scores, and splenic T-lymphocyte phenotype. Finally, T-lymphocyte KLF3 expression was detected through flow cytometry. Results: Over the 7 days of observation, septic mice demonstrated 64.7%… More >

JYOTHI B. NAIR^1,2, ANU MARY JOSEPH³, SANOOP P.⁴, MANU M. JOSEPH^5,*

BIOCELL, Vol.48, No.11, pp. 1579-1602, 2024, DOI:10.32604/biocell.2024.054879 - 07 November 2024

Abstract Nanoparticles represent a heterogeneous collection of materials, whether natural or synthetic, with dimensions aligning in the nanoscale. Because of their intense manifestation with the immune system, they can be harvested for numerous bio-medical and biotechnological advancements mainly in cancer treatment. This review article aims to scrutinize various types of nanoparticles that interact differently with immune cells like macrophages, dendritic cells, T lymphocytes, and natural killer (NK) cells. It also underscores the importance of knowing how nanoparticles influence immune cell functions, such as the production of cytokines and the presentation of antigens which are crucial for… More >

Gloria B. Kim^1,†, Qiong Wei^2,†, Virginia Aragon-Sanabria¹, Sulin Zhang², Jian Yang¹, Cheng Dong^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 15-15, 2019, DOI:10.32604/mcb.2019.07137

Abstract Most cells survive and grow by attaching and spreading on a substrate. They generate internal tension that contracts the cell body and thus exert tractions on the underlying substrate through focal adhesions. Traction force also plays a critical role in many biological processes, such as inflammation, metastasis, and angiogenesis. Thus, measuring the cell traction force provides valuable information on understanding the underlying mechanism of these biological processes. Here, a traction force microscopy (TFM) method using super thin hydrogels composed of immobilized fluorescent beads was utilized to quantify the mechanical forces generated during the transmigration of… More >

Patrycja Libako¹, Julia Miller¹, Wojciech Nowacki¹, Sara Castiglioni², Jeanette A. Maier², Andrzej Mazur³

European Cytokine Network, Vol.26, No.1, pp. 1-9, 2015, DOI:10.1684/ecn.2015.0361

Abstract Magnesium is highly involved in the metabolic network such that even subtle disturbances in its homeostasis affect many cellular functions, including calcium homeostasis, signal transduction, energy metabolism, membrane stability and cell proliferation. Recently, magnesium level has been proposed to modulate the priming and activity of immune cells. We studied the behavior of antigen-presenting cells (APCs) and T lymphocytes after altering the magnesium/calcium balance. We used two different populations of primary APCs, i.e. bone marrow-derived dendritic cells and bone marrow-derived macrophages, while D10.G4.1 cells served as a model of responding Th2 cells. Our principal findings are More >

Knut Liseth¹, Malvin Sjo², Kristin Paulsen³, Øystein Bruserud^2,3, Elisabeth Ersvaer³

European Cytokine Network, Vol.21, No.1, pp. 40-49, 2010, DOI:10.1684/ecn.2009.0181

Abstract Previous studies of T cell reconstitution following allogeneic stem cell transplantation have described long-lasting T cell defects, including decreased levels of autocrine proliferating CD4+ T cells. However, T cell functions during the early phase of conditioning-induced, pre-engraftment pancytopenia have not been characterized previously. We used a whole blood assay to investigate T cell proliferation and cytokine release during the period of pre-engraftment cytopenia. The study included 13 acute leukemia patients receiving mye-loablative conditioning followed by transplantation of G-CSF-mobilised peripheral blood stem cells derived from HLA-matched family donors. Maximal proliferation and cytokine release could not be… More >

Ursula Azizi-Semrad^1,*, Dagmar Krenbek^2,*, Günther Hofbauer¹, Georgios Karanikas³, Eduardo Maldonado-Gonzalez¹, Peter Pietschmann¹, Martin Willheim^1,4

European Cytokine Network, Vol.21, No.2, pp. 105-115, 2010, DOI:10.1684/ecn.2010.0190

Abstract The number of functional subsets of CD4+ T lymphocytes distinguished by their cytokine produc-tion has been extended in the last decade. The in vitro generation of a T cell subset characterized by IL-6production has resurrected the question of cytokine co-expression patterns in T cells. In order to delineatethese cells as a specific functional subpopulation in vivo, we profiled the cytokine production pattern of humanperipheral blood CD4+ T lymphocytes across established subsets. We provide evidence for a new T cell subsetTh6, with an IL-6 signature. Freshly isolated PBMC were analyzed using intracellular cytokine detection(IDC). Cytokine co-expression… More >

IVÓN TERESA NOVAK, HUMBERTO RAMÓN CABRAL

BIOCELL, Vol.32, No.2, pp. 169-174, 2008, DOI:10.32604/biocell.2008.32.169

Abstract We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected More >