RAN PAN^1,#, JIAYAN MAO^2,#, YUELIANG ZHENG³, WEI CHEN², JUNPING GUO^1,*, LIJUN WANG^3,*

BIOCELL, Vol.48, No.3, pp. 431-441, 2024, DOI:10.32604/biocell.2023.046484 - 15 March 2024

Abstract Background: Sepsis, a type of systemic disease, can impact nearly all organs, tissues and cells. Among them, endothelial cells are amongst the first to be affected and respond to the insult. In this study, we investigated the protective effects of microRNA-30a-5p (miR-30a-5p) on human pulmonary microvascular endothelial cells (HPMECs) treated with lipolysaccharide (LPS). Methods: An in vitro model of sepsis was established in HPMECs with the use of LPS. Transfecting with different tools (mimetic and inhibitor) to modify miR-30a-5p expression. Cell viability, proliferation and apoptosis were detected by the CCK-8 assay, the EdU kit and fluorescence… More >

Hong Liu^1,#,*, Luyao Ma^1,#, Jinghang Li^1,#, Bingqi Sun², Siqiang Zheng³, Yongfeng Shao^1,*

Congenital Heart Disease, Vol.16, No.5, pp. 443-455, 2021, DOI:10.32604/CHD.2021.015588 - 03 June 2021

Abstract Background: This study evaluated differential inflammatory response to cardiopulmonary bypass reoxygenation in tetralogy of Fallot repair. Methods: We performed a retrospective study at a cardiovascular center from 2012 to 2018, including 500 patients aged 1 week–18 years who received complete repair of tetralogy of Fallot. Patients were grouped according to tertiles of preoperative RVOT gradient on echocardiography into mild, moderate, and severe stenosis. We measured the highest perfusate oxygenation (PpO2) during aortic occlusion as independent variable. Primary outcome was systemic inflammatory response syndrome (SIRS) within 7 days postoperatively or the time of death or discharge. Results: Overall, rate… More >