Shengchao Zhang, Jun Yuan, Ruheng Zheng

Oncology Research, Vol.24, No.4, pp. 263-269, 2016, DOI:10.3727/096504016X14666990347392

Abstract Recently, deubiquitinating enzymes (DUBs) are emerging as new regulators in cancer progression. However, understanding of the involvement of DUBs in non-small cell lung cancer (NSCLC) is just beginning. In this study, we investigated the expression and biological function of ubiquitin-specific peptidase 17 (USP17) in NSCLC progression in vitro and in vivo. We found that the expression of USP17 was higher than in a normal control. We further efficiently depleted USP17 expression in two different NSCLC cells, A549 and H1299. The anchorage-independent growth ability of these cells, estimated by soft agar colony formation assay, was significantly More >

Guoqiang Zhang^*1, Zengyan Liu^†1, Yong Han^*, Xiaohong Wang^*, Zhenlin Yang^*

Oncology Research, Vol.24, No.4, pp. 233-238, 2016, DOI:10.3727/096504016X14648701447977

Abstract Triple-negative breast cancer (TNBC) is associated with high recurrence rates of metastasis and death. miR-509 has been reported to be a tumor suppressor in many cancers, but its effect in TNBC has not yet been identified. In this article, we explored the effects of miR-509 on the malignant phenotype of TNBC cells, including proliferation, apoptosis, migration, and invasion. We transiently transfected TNBC cells, Hs578T, with miR-509 mimic. Upon transfection, the expression of miR-509 was upregulated about 50-fold compared with cells transfected with scramble mimic. Overexpression of miR-509 inhibited cell proliferation, induced cell apoptosis, and suppressed More >

Ting Zhang¹, Lei Ye¹, Lingfei Han, Qizhi He, Jianlong Zhu

Oncology Research, Vol.24, No.3, pp. 189-196, 2016, DOI:10.3727/096504016X14641336229602

Abstract Ovarian cancer is highly malignant with a gradually increasing incidence and a high mortality rate. Immunosuppression is induced in ovarian cancer, although the mechanism detail is not clear. It has been indicated that HVEM (herpesvirus entry mediator) B- and T-lymphocyte attenuator (BTLA) negatively regulates the immune responses of T lymphocytes. Here, HVEM mRNA was found to be elevated in ovarian cancer tissue samples and primary ovarian cancer cells in comparison with benign tissue samples. We then knocked down HVEM expression in an ovarian cancer cell line, OVCAR3, by lentivirus-based small hairpin RNA (shRNA). Cell Counting… More >

Ting Zhang¹, Lei Ye¹, Qizhi He, Jianlong Zhu

Oncology Research, Vol.25, No.9, pp. 1665-1665, 2017, DOI:10.3727/096504017X15078984695565

Abstract Ovarian cancer is highly malignant with a gradually increasing incidence and a high mortality rate. Immunosuppression is induced in ovarian cancer, although the mechanism detail is not clear. It has been indicated that HVEM (herpesvirus entry mediator) B- and T-lymphocyte attenuator (BTLA) negatively regulates the immune responses of T lymphocytes. Here, HVEM mRNA was found to be elevated in ovarian cancer tissue samples and primary ovarian cancer cells in comparison with benign tissue samples. We then knocked down HVEM expression in an ovarian cancer cell line, OVCAR3, by lentivirus-based small hairpin RNA (shRNA). Cell Counting… More >

Cheng Xu^*1, Lu Cao^*1, Jianhua Liu^†1, Zhongrun Qian^‡, Yu Peng^§, Wenjing Zhu^§, Yongming Qiu^‡, Yingying Lin^‡¶

Oncology Research, Vol.25, No.3, pp. 407-415, 2017, DOI:10.3727/096504016X14743350548249

Abstract Elevated circulating asparaginyl endopeptidase (AEP), a novel lysosomal protease, has been found in breast cancer, and AEP is thus considered to be a prognostic factor in this disease. However, the pathological functions of circulating AEP in the development of breast cancer and the potential of AEP-targeted therapy remain unclear. We used MMTV-PyVmT transgenic mice, which spontaneously develop mammary tumors. Western blotting showed overexpression of AEP in both primary tumor tissue and lung metastases compared to their normal counterparts. Moreover, the concentration of circulating AEP gradually increased in the serum during the development of mammary tumors. More >

Qianren Xiao^*1, Lu Huang^†1, Zhongzu Zhang^‡1, Xiang Chen^*, Jiaquan Luo^*, Zhanmin Zhang^§, Shaoqing Chen^§, Yong Shu^*, Zhimin Han^*, Kai Cao^*

Oncology Research, Vol.25, No.2, pp. 267-275, 2017, DOI:10.3727/096504016X14732510786564

Abstract miRNAs play a pivotal role in the development and progression of osteosarcoma (OS). Previous studies indicated that miR-140 acts as a tumor suppressor in many cancers. However, its accurate expression and exact function in OS cells remain unknown. Herein, we demonstrated the lower expression of miR-140 in 40 paired OS tissues. Restoring miR-140 expression in OS cells had a marked effect on inhibiting cell proliferation and invasion, inducing cell apoptosis in vitro, and suppressing tumor growth in vivo. Moreover, a bioinformatics prediction indicated that the histone deacetylase 4 (HDAC4) is a target gene of miR-140 and More >

Hui Zhou^*†, Yanglin Liu^†, Ling Xiao^‡, Zhengmao Hu^*, Kun Xia^*§

Oncology Research, Vol.25, No.1, pp. 147-154, 2017, DOI:10.3727/096504016X14732772150505

Abstract MicroRNA-27b (miR-27b) was recently found to be significantly downregulated in different human cancers. However, evidence of the function of miR-27b in non-small cell lung cancer (NSCLC) remains limited. In this study, we aimed to investigate novel miR-27b-mediated targets or signaling pathways associated with the tumorigenesis and metastasis of NSCLC. Real-time (RT) PCR was performed to examine miR-27b expression in NSCLC specimens. MTT assay, wound-healing assay, and Transwell assay were used to determine cell proliferation, migration, and invasion. Our data indicated that the miR-27b levels were significantly decreased in NSCLC specimens and cell lines (SK-MES-1, H358,… More >

Xianbiao Shi, Xiaoqiao Tang, Lei Su

Oncology Research, Vol.26, No.6, pp. 869-878, 2018, DOI:10.3727/096504017X15123838050075

Abstract This study aimed to investigate the effect of long noncoding RNA PTENP1 in the development of breast cancer (BC). Quantitative real-time PCR was utilized to determine the expression of PTENP1 in tissues and cell lines. pcDNA3.1 and shRNA were used to over- and low-express PTENP1 in BC cell lines, and miR-19b mimic and inhibitor were utilized to over- and low-express miR-19b. Then the abilities of cell survival, apoptosis, migration, and invasion were assessed in BC cells with different expression levels of PTENP1 and miR-19b. The expression of PTENP1 was significantly downregulated in both BC tissues More >

Yingchao Li^*, Xiaoni Yan^*, Li Ren^*, Yang Li^†

Oncology Research, Vol.26, No.1, pp. 1-8, 2018, DOI:10.3727/096504016X14772410356982

Abstract Epithelial–mesenchymal transition (EMT) is one of the most important mechanisms in the metastasis of various cancers, including gastric cancer (GC). In this study, we explored the putative significance of miR-644a and its role in EMT-mediated metastasis of GC. We first detected the expression of miR-644a in a cohort of 107 GC tissues using quantitative RT-PCR. The expression of miR-644a was suppressed in GC tissues and was associated with a later clinical stage and tumor metastasis. Restoring the expression of miR-644a could significantly suppress the migration and invasion of HGC-27 and SGC-7901 cells, which might be More >

Haitao Liu^1,2,*, Jiaming Wang¹, Xiuliang Zhang¹, Yanji Jiang², Qian Xiao¹

CMES-Computer Modeling in Engineering & Sciences, Vol.139, No.3, pp. 2747-2772, 2024, DOI:10.32604/cmes.2024.047129

Abstract The expansion chamber serves as the primary silencing structure within the exhaust pipeline. However, it can also act as a sound-emitting structure when subjected to airflow. This article presents a hybrid method for numerically simulating and analyzing the unsteady flow and aerodynamic noise in an expansion chamber under the influence of airflow. A fluid simulation model is established, utilizing the Large Eddy Simulation (LES) method to calculate the unsteady flow within the expansion chamber. The simulation results effectively capture the development and changes of the unsteady flow and vorticity inside the cavity, exhibiting a high… More > Graphic Abstract