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  • Open Access

    REVIEW

    Revolutionizing stem cell research: unbiased insights through single-cell sequencing

    HAO WU#, NA HUO#, SITUO WANG, ZIWEI LIU, YI JIANG*, QUAN SHI*

    BIOCELL, Vol.48, No.11, pp. 1531-1542, 2024, DOI:10.32604/biocell.2024.054278 - 07 November 2024

    Abstract Stem cells have shown great application potential in wound repair, tissue regeneration, and disease treatment. Therefore, a full understanding of stem cells and their related regulatory mechanisms in disease treatment is conducive to improving the therapeutic effect of stem cells. However, thus far, there are still many unsolved mysteries in the field of stem cells due to technical limitations, which hinder the in-depth exploration of stem cells and their wide clinical application. Single-cell sequencing (SCS) has provided very powerful and unbiased insights into cell gene expression profiles at the single-cell level, bringing exciting results to More >

  • Open Access

    ARTICLE

    Immature oocyte quality and maturational competence of porcine cumulus-oocyte complexes subpopulations

    GABRIEL MARTIN ALVAREZ1*, GABRIEL CARLOS DALVIT1, MARÍA VERÓNICA ACHI1, MARCELO SERGIO MIGUEZ2, PABLO DANIEL CETICA1

    BIOCELL, Vol.33, No.3, pp. 167-177, 2009, DOI:10.32604/biocell.2009.33.167

    Abstract Porcine immature oocyte quality (i.e., that of live oocytes at the germinal vesicle stage) was evaluated according to features of the surrounding cumulus, aiming to establish maturational competence of different subpopulations of such cumulus-oocyte complexes. Six subpopulations were identified: A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (partly naked oocytes), C (naked oocytes), D (with a dark cumulus). The percent incidence of live oocyte in these subpopulations changed significantly as related to cumulus features, however the occurrence of oocytes in the germinal vesicle stage was lower in class D… More >

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