Djordje P. Božović^1,2, Anja Rimac³, Milorad M. Vujičić^1,4, Pragya Singh⁵, Michal Goga⁵, Mingai Li^2,6, Claudio Varotto^2,6, Aneta D. Sabovljević^1,4, Marko S. Sabovljević^1,4,5,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2949-2961, 2024, DOI:10.32604/phyton.2024.057995 - 30 November 2024

Abstract Physcomitrium eurystomum Sendtn. is a very rare European ephemeral funaroid moss. The entire European population of this species is considered threatened and it is red-listed in many regions and countries. In addition to being recognized as threatened and included in nature conservation legislation, it also requires active protection measures. This study aims to contribute to effective conservation practices for P. eurystomum. Different conservation physiology tests were carried out to propagate this species to achieve a reliable procedure for biomass production and the potential reintroduction of germplasm. Ex situ tests, both in vitro and ex vitro, were carried out to determine… More >

Yuebo Jing^*, Jihua Mao, Rongbo Li

Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2719-2732, 2022, DOI:10.32604/phyton.2022.021989 - 29 August 2022

Abstract Olive (Olea europaea L.) is one of the most important and widely cultivated fruit trees, with high economic, ecological, cultural and scientific value. China began introducing and cultivating olive in the 1960s, and Yunnan Province is one of the main growing areas. Improving the cultivation and productivity of this tree crop species is an important challenge. Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi (AMF) for this plant is well recognized; nevertheless, studies of olive AMF in China are still very limited. Roots and rhizosphere soils of olive were sampled from… More >

YUNHUI ZHOU, YAJIE LIU, WENNA ZHAO, FEI CHEN, YUDAN WANG, CHUNXUE YANG

BIOCELL, Vol.46, No.11, pp. 2465-2474, 2022, DOI:10.32604/biocell.2022.021016 - 07 July 2022

Abstract Salinization has led to the deterioration of the ecological environment, affected the growth of plants, and hindered the development of agriculture and forestry. Arbuscular mycorrhizal (AM) fungi, as important soil microorganisms, play significant physiological and ecological roles in promoting plant nutrient absorption and improving soil structure. Puccinellia tenuiflora (Turcz.) Scribn. et Merr. in Songnen saline-alkaline grassland was selected as the research object to observe AM fungal colonization of the roots and explore the species and diversity of AM fungi in symbiotic association with P. tenuiflora. This study showed that AM fungi colonized in P. tenuiflora roots and formed… More >

Longhua Zhou^1,2,#, Ting He^1,2,#, Jing Li^1,2,3, Guimei Guo^1,2, Yingbo Li^1,2, Hongwei Xu^1,2, Runhong Gao^1,2, Linli Huang^1,2, Yifei Wang^1,2, Ruiju Lu^1,2, Zhiwei Chen^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912 - 14 March 2022

Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were More >

Yingbo Li^1,2,#, Ting He^1,2,#, Guimei Guo^1,2, Hongwei Xu^1,2, Yingjie Zong^1,2, Shuwei Zhang^1,2, Ruiju Lu^1,2, Longhua Zhou^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1153-1164, 2022, DOI:10.32604/phyton.2022.019520 - 14 February 2022

Abstract Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process. Hua 30 MDEC can be successfully infected by Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from… More >

Wuzhong Yin^#, Hongxia Yang^#, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua He^*, Nan Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 727-744, 2022, DOI:10.32604/phyton.2022.017811 - 09 December 2021

Abstract The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant. The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the More >

Chunxue Yang^*, Yajie Liu, Wenna Zhao, Na Wang

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 719-729, 2021, DOI:10.32604/phyton.2021.015024 - 30 March 2021

Abstract To understand arbuscular mycorrhizal (AM) fungi resources and develop AM fungal species in ornamental plants with saline-alkaline tolerances, Iris lactea, which grows in the Songnen saline-alkaline grassland with a high ornamental value, was selected as the experimental material, and the colonization characteristics of its roots and the AM fungal diversity in its rhizosphere were explored. The results of the observations and calculations of mycorrhizae from ten different samples showed that AM fungi colonized the roots of I. lactea and formed Arum-type mycorrhizal structures. There was a significant correlation between soil spore density and pH value, while the colonization… More >

María Laura Molinelli^*, Rocío Tarifa, Patricia Perissé

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 345-360, 2020, DOI:10.32604/phyton.2020.08796 - 22 April 2020

Abstract Echium plantagineum (Boraginaceae) is native of the Mediterranean regions, has been introduced and become widespread within the American continent (North to South), South Africa, New Zealand and Australia. This plant has seed dormancy, aggressively spreads to infest vast areas of predominantly agricultural land and is considered a toxic weed to livestock. The objectives of this research were: (i) to study the morpho-anatomy of the diaspores of E. plantagineum; (ii) to identify the pathway of water uptake; and (iii) to characterize the germination and the seedling. The morpho-anatomical studies were carried out analyzing semi-permanent slides of transverse and… More >

Silvana L. D’AMICO¹, Patricia I. LEONARDI ^1,2*, Eduardo J. CÁCERES¹

BIOCELL, Vol.37, No.3, pp. 85-90, 2013, DOI:10.32604/biocell.2013.37.085

Abstract This is the first comprehensive ultrastructural study on dwarf males with external gametangia in the ge in the genus Oedogonium, from androspore germination to the liberation of mature male gametes. The ultrastructure of the process in O. macrandrium Wittrok is similar to that of Bulbochaete hiloensis (Nordstedt) Tiffany, butwith two remarkable differences. In O. macrandrium : 1) instead of a true transverse wall, only condensed mucilage appears between the gametes of each antheridial cell, and 2) the cell wall between the basal cell and the basal most antheridial cell has simple plasmodesmata similar to those More >

Bonomo MC, OG Martínez, ME Tanco, R Cardozo, Z Avilés

Phyton-International Journal of Experimental Botany, Vol.82, pp. 119-126, 2013, DOI:10.32604/phyton.2013.82.119

Abstract The tree fern Alsophila odonelliana is found from southern Bolivia to northwestern Argentina, in the Tucuman-Bolivian forests. Spore germination was tested in three sterile nutritive media: Dyer (D), Murashige and Skoog (MS) and Knudson (K). We also evaluated the effect on gametophyte development of the addition of two growth regulators, BAP and 2,4-D to the culture medium Dyer; 2,4-D was evaluated using two concentrations: 0.1 and 2.0 mg/L, and BAP using three concentrations, 0.01; 0.1 and 1.0 mg/L. We found no significant differences in the germination of spores among all three media. We noted that supplementing More >