SAYUMI TAHARA¹, SYDNEY RENTSCH¹, FERNANDA COSTAS CASAL DE FARIA¹, PATRICIA SARCHET¹, ROMA KARNA¹, FEDERICA CALORE^2,*, RAPHAEL E. POLLOCK¹

Oncology Research, Vol.33, No.1, pp. 1-13, 2025, DOI:10.32604/or.2024.053635 - 20 December 2024

Abstract Liposarcoma is one of the most common soft tissue sarcomas, however, its occurrence rate is still rare compared to other cancers. Due to its rarity, in vitro experiments are an essential approach to elucidate liposarcoma pathobiology. Conventional cell culture-based research (2D cell culture) is still playing a pivotal role, while several shortcomings have been recently under discussion. In vivo, mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture. However, they do not fully recapitulate human dedifferentiated liposarcoma (DDLPS) characteristics. Therefore, three-dimensional (3D) culture systems have been the recent… More >

SANDRA STICKLER¹, BARBARA RATH¹, MAXIMILIAN HOCHMAIR², CLEMENS LANG³, LUKAS WEIGL⁴, GERHARD HAMILTON^1,*

Oncology Research, Vol.31, No.1, pp. 13-22, 2023, DOI:10.32604/or.2022.027281 - 01 March 2023

Abstract Small cell lung cancer (SCLC) is frequently disseminated and has a dismal prognosis with survival times of approximately two years. This cancer responds well to initial chemotherapy but recurs within a short time as a globally chemoresistant tumor. Circulating tumor cells (CTCs) are held responsible for metastasis, the extremely high numbers of these cells in advanced SCLC allowed us to establish several permanent CTC cell lines. These CTCs are distinguished by the spontaneous formation of large spheroids, termed tumorospheres, in regular tissue culture. These contain quiescent and hypoxic cells in their interior and are associated… More >

HONGYANG LI^1,2, CHAN WANG⁴, SHIWEI LIU¹, YONGLONG GUO^1,3,4,*, JIANSU CHEN^1,3,4,*

BIOCELL, Vol.46, No.7, pp. 1705-1716, 2022, DOI:10.32604/biocell.2022.018442 - 17 March 2022

Abstract Human adipose stem cells (hADSCs) are reliable sources for cell therapy. However, the clinical applications are limited by the decrease in activity during in vitro culture. We used a knockout serum replacement (KSR) medium, Eppendorf (EP) tube culture, and a simulated microgravity (SMG) culture system to establish hADSC spheroids. We found that hADSCs aggregated and formed spheroids in the KSR culture medium. The EP tube culture method revealed many biological cell characteristics, such as good cell viabilities, rough surfaces, polar growth, fusion phenomenon, and injectability. The findings show its advantages for hADSCs spherical cultures. When cultured… More >

Kalaivani Muniandy¹, Zuhaida Asra Ahmad^1,4, Sylvia Annabel Dass¹, Shaharum Shamsuddin², Nethia Mohana Kumaran³, Venugopal Balakrishnan^1,*

Oncologie, Vol.23, No.2, pp. 279-291, 2021, DOI:10.32604/Oncologie.2021.015969 - 22 June 2021

Abstract Spheroids are generally self-assembled cells with the ability to generate their extracellular matrix, including the complex cell-matrix and the cell-cell interactions that resemble the functional characteristics of the corresponding tissue in vivo. The study aimed to develop a three-dimensional (3D) spheroid system for the cervical cancer cell lines, HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), C33A (non-HPV), HT3 (non-HPV) as well as to identify its biological activity in the extracellular form. For the formation of the cervical cancer spheroids, the liquid overlay approach was applied, followed by embedding to the bovine collagen I matrix. Spheroid formation using More >

M. Krishna Prasad¹, G. Manpreet Kaur¹

FDMP-Fluid Dynamics & Materials Processing, Vol.13, No.2, pp. 107-125, 2017, DOI:10.3970/fdmp.2017.013.107

Abstract The slow steady rotation of a micropolar fluid spheroid whose shape deviates slightly from that of a sphere in concentric spheroidal container filled with Newtonian viscous fluid is studied analytically. The boundary conditions used are the continuity of velocity and stress components, and spin vorticity relation. The torque and wall correction factor exerted on the micropolar fluid spheroid is obtained. The dependence of wall correction factor on the micropolarity parameter, spin parameter, viscosity ratio and deformation parameter is studied numerically and its variation is presented graphically. In the limiting cases, the torque acting on solid More >

Hong Y. Yeh, Huan J. Keh^{1, *}

CMES-Computer Modeling in Engineering & Sciences, Vol.113, No.3, pp. 343-366, 2017, DOI:10.3970/cmes.2017.113.361

Abstract The problem of the steady migration of an axially symmetric prolate particle along its axis of revolution coinciding with the centerline of a circular capillary is investigated semi-analytically in the limit of low Reynolds number, where the viscous fluid may slip at the solid surfaces. A method of distribution of spherical singularities along the axis inside the particle is employed to establish the general solution of the fluid velocity satisfying the boundary conditions at the capillary wall and infinity. The slip condition at the particle surface is then satisfied by using a boundary collocation method… More >

Yi W. Wan¹, Huan J. Keh²

CMES-Computer Modeling in Engineering & Sciences, Vol.74, No.2, pp. 109-138, 2011, DOI:10.3970/cmes.2011.074.109

Abstract The steady rotation of an axially symmetric particle about its axis of revolution normal to two plane walls at an arbitrary position between them in a viscous fluid is studied theoretically in the limit of small Reynolds number. The fluid is allowed to slip at the surface of the particle. A method of distribution of a set of spherical singularities along the axis of revolution inside a prolate particle or on the fundamental disk within an oblate particle is used to find the general solution for the fluid velocity distribution that satisfies the boundary conditions… More >

Huan J. Keh¹, Yu C. Chang²

CMES-Computer Modeling in Engineering & Sciences, Vol.62, No.3, pp. 225-254, 2010, DOI:10.3970/cmes.2010.062.225

Abstract A theoretical study of the Stokes flow caused by a rigid particle of revolution translating axisymmetrically perpendicular to two parallel plane walls at an arbitrary position between them in a viscous fluid, which may slip at the particle surface, is presented. A method of distribution of a set of spherical singularities along the axis of revolution within a prolate particle or on the fundamental plane within an oblate particle is used to find the general solution of the fluid velocity field that satisfies the boundary conditions at the plane walls and at infinity. The slip… More >

A. Vukovic¹, P. Sewell¹, T. M. Benson¹

CMES-Computer Modeling in Engineering & Sciences, Vol.55, No.2, pp. 171-190, 2010, DOI:10.3970/cmes.2010.055.171

Abstract A fast and accurate method is developed for the analysis of a class of metal three-dimensional resonators with rotational symmetry. The analysis is formulated using the Body of Revolution approach and the Method of Analytical Regularization. This development is motivated by the need for three-dimensional analytical solvers that could enable fast and accurate analysis of photonic resonant structures which support very high Q whispering gallery modes and which are computationally challenging for numerical simulations. The paper outlines the formulation of the method and demonstrates the stability and the source of computation errors of the method. More >

Yi W. Wan¹, Huan J. Keh²

CMES-Computer Modeling in Engineering & Sciences, Vol.53, No.1, pp. 73-94, 2009, DOI:10.3970/cmes.2009.053.073

Abstract The problem of the rotation of a rigid particle of revolution about its axis in a viscous fluid is studied theoretically in the steady limit of low Reynolds number. The fluid is allowed to slip at the surface of the particle. A singularity method based on the principle of distribution of a set of spherical singularities along the axis of revolution within a prolate particle or on the fundamental plane within an oblate particle is used to find the general solution for the fluid velocity field that satisfies the boundary condition at infinity. The slip… More >