GUIBIN ZHONG^1,2,#, SHURONG WANG^3,#, YUJIN HE⁴, DAMING FENG¹, KE WEI¹, YANQIU YANG¹, JIANWEI CHEN^1,2,*, JUNLING CHEN^1,*

BIOCELL, Vol.48, No.6, pp. 1001-1008, 2024, DOI:10.32604/biocell.2024.050294

Abstract Background: Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. Methods: Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR-155/sirtuin 1 More >

Shi Ying^*, Huang Jianjun^†, Yi Xue^*, Yu Shuwei^*, Zhang Liyuan^*, Wang Jie^*, Cheng Lixian^*

Oncology Research, Vol.25, No.9, pp. 1421-1430, 2017, DOI:10.3727/096504016X14826089198805

Abstract MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a More >

Daofu Jia^*, Yanping Niu^†, Dongling Li^‡, Zhaorui Liu^§

Oncology Research, Vol.26, No.5, pp. 753-764, 2018, DOI:10.3727/096504017X15024946480113

Abstract Osteosarcoma is a highly aggressive malignant bone tumor with poor prognosis. Evidence has suggested that lncRNAs are deregulated in multiple cancers. In this study, we investigated the role of the lncRNA C2dat1 on the biological functions of osteosarcoma cells. The expressions of C2dat1, miR-34a-5p, and Sirt1 in human osteosarcoma cells were altered by transfection with their specific vectors/shRNA or mimic/inhibitor. Cell viability, migration, invasion, and apoptosis were assessed posttransfection. The mRNA and protein levels of C2dat1, miR-34a-5p, and Sirt1 were detected by qRT-PCR and Western blot. The results showed that C2dat1 suppression reduced cell viability, More >

Lei Ou^*, Dazhong Wang^†, Han Zhang^*, Qian Yu^*, Fangfang Hua^‡

Oncology Research, Vol.26, No.3, pp. 401-410, 2018, DOI:10.3727/096504017X15017209042610

Abstract MicroRNAs (miRNAs) play important roles in the carcinogenesis of cervical cancer. However, the expression and underlying mechanisms of miRNA in cervical cancer progression remain unclear. In the present study, our data showed that the expression of miR-138-5p was significantly downregulated in cervical cancer tissues, and decreased expression of miR-138-5p was correlated with advanced FIGO stage, poor differentiation, lymph node metastasis, and poor overall survival of cervical cancer patients. Function assays showed that overexpression of miR-138-5p reduced cervical cancer cell proliferation, arrested cells in the G₀ /G₁ phase, and induced cell apoptosis in vitro. Remarkably, SIRT1 was More >

MIN SHEN^1,2, XIAOMAN XU^1,2, GUANGLING SUN¹, LIANGZHU WANG¹, TAO YING¹, HANG SU¹, WEI WANG¹, QINGHUA CAO^1,*, ZHEZHE SUN^1,*

BIOCELL, Vol.48, No.3, pp. 491-499, 2024, DOI:10.32604/biocell.2023.047640

Abstract Objective: Circular RNAs (circRNAs) have been shown to involve in pathological processes of ischemic stroke (IS), including autophagy. This study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in IS and the related mechanisms. Methods: Expression of circR-ZC3HC1 in blood samples of IS patients and healthy controls was detected. Hippocampal neurons were treated with oxygen and glucose deprivation (OGD) to establish IS in vitro model. The expression of LC3 and p62 and the number of autophagosomes were examined to evaluate the autophagy level of OGD induced neurons using western blotting and transmission electron… More >

LONGJU QI^1,2,#, XIAOYING XU^3,#, BIN LI^4,#, BO CHANG⁵, SHENGCUN WANG², CHUN LIU², LIUCHENG WU², XIAODI ZHOU⁴, QINGHUA WANG^2,*

BIOCELL, Vol.46, No.4, pp. 989-998, 2022, DOI:10.32604/biocell.2022.018014

Abstract Excessive fat ectopically deposited in the non-adipose tissues is considered as one of the leading causes of myopathy. The aim of this study was to investigate the role of Dihydroartemisinin (DHA) in palmitate (PAL)-incubated H9c2 cells (lipotoxicity-induced cell injury model). Cell viability of PAL-treated cells was determined by MTT assay, and apoptotic regulators were examined by qRT-PCR and western blot analysis, in the absence or in the presence of DHA, respectively. Expression levels of miR-133b and Sirt1 were also evaluated by qRT-PCR and western blotting examination. PAL decreased the viability of H9c2 cells and enhanced More >

ATTALLA F. EL-KOTT^1,2,*, AYMAN E. EL-KENAWY³, EMAN R. ELBEALY⁴, ALI S. ALSHEHRI¹, HEBA S. KHALIFA², MASHAEL MOHAMMED BIN-MEFERIJ⁵, EHAB E. MASSOUD^6,7,8, AMIRA M. ALRAMLAWY⁹

BIOCELL, Vol.45, No.5, pp. 1337-1353, 2021, DOI:10.32604/biocell.2021.015464

Abstract This study examined if the anti-tumorigenesis effect of Exendin-4 in HT29 and HCT116 colorectal cancer (CRC) involves modulation of SIRT1 and Akt/GSR3K/β-catenin/NF-κB axis. HT29 and HCT116 cells were treated either with increasing levels of Exendin-4 (0.0-200 µM) or with Exendin-4 (at its IC₅₀) in the presence or absence of EX-527 (10 µM/a selective SIRT1 inhibitor) or Exendin-4 (9-39) amide (E (9-39) A) (1 µM/an Exendin-4 antagonist). In a dose-dependent manner, Exendin-4 inhibited cell survival, but enhanced levels of lactate dehydrogenase (LDH) and single-stranded DNA (ssDNA) in both HT29 and HCT116. In both cell lines and at… More >

YUN SUN^#, YI LIU^#, BINHUA XUE, XIAODIE WANG, WEILI YU^*

BIOCELL, Vol.45, No.1, pp. 129-138, 2021, DOI:10.32604/biocell.2021.013275

Abstract Hyperlipidemia acute pancreatitis (HLAP) is a significant cause of AP, characterized by recurrent attacks, more complications and high incidence and mortality. HLAP is often accompanied by single or multiple organ damage. Negative regulation of interferon-regulatory factor 9 (IRF9) on sirtuin-1 (SIRT1) contributes to a range of diseases. However, the function of IRF9 and SIRT1, and the relationship of the two in HLAP with heart injury remain to be illustrated so far. Animal models of HLAP were set up by feeding with high-fat chow and subsequently injecting 20% L-arginine intraperitoneally. The degree of pancreas and heart… More >

HELIN FENG^1,2, XIAOCHONG ZHANG³, QIANQIAN ZHANG⁴, ZE LI⁵, LILI ZHAO^1,6,*

BIOCELL, Vol.44, No.3, pp. 443-450, 2020, DOI:10.32604/biocell.2020.010705

Abstract Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis of OS remains poor due to early metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fully understood. In this study, we used a human osteosarcoma MG-63 cell line as an experimental model. MG-63 cells were treated with leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed that leptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover, the expression of More >

XINGXING ZHU^1,2, TIANFENG HUA^1,2, MINGFEI WU³, JIATIAN WU^1,2, JIANCHAO HONG^1,2, MIN YANG^1,2,*

BIOCELL, Vol.44, No.3, pp. 431-441, 2020, DOI:10.32604/biocell.2020.010323

Abstract Post-resuscitation myocardial dysfunction (PRMD) is the most severe myocardial ischemia-reperfusion injury (MIRI) and is characterized by difficult treatment and poor prognosis. Research has shown the protective effects of the rational use of ivabradine (IVA) against PRMD; however, the molecular mechanisms of IVA remain unknown. In this study, an ischemia-reperfusion injury (IRI) model was established using hypoxic chambers. The results demonstrated that pretreatment with IVA reduced IRI-induced cytotoxicity and apoptosis. IVA attenuated mitochondrial damage, eliminated excess reactive oxygen species (ROS), suppressed IRI-induced ATP and NAD⁺ , and increased the AMP/ATP ratio. We further found that IVA increased More >