YANGYI HE^1,2, DAVID SANCHEZ-LORENTE^3,4,5, MELISSA ACOSTA-PLASENCIA¹, MARC BOADA^3,4,5, ANGELA GUIRAO^3,4,5, RAMON M. MARRADES^4,5,6,7, LAUREANO MOLINS^3,4,5, ALFONS NAVARRO^1,4,5,*

BIOCELL, Vol.47, No.5, pp. 951-957, 2023, DOI:10.32604/biocell.2023.027718

Abstract Tumor-secreted extracellular vesicles (EVs) participate in the metastasis process through different mechanisms, including the preparation of the pre-metastatic niche to grant circulating tumor cells (CTCs) implantation and growth. The study of the metastasis process through the analysis of CTCs and tumor-derived EVs is difficult because of the dilution grade of these elements in peripheral blood. In early-stage lung cancer patients, the tumor-secreted products are even more diluted. An attractive strategy in surgical lung cancer patients is to purify them from a pulmonary tumor-draining vein where they are enriched. The information obtained from the analysis of EVs and CTCs purified from… More >

JÉRÔME LALOZE^1,2, ALEXIS DESMOULIÈRE^1,*

BIOCELL, Vol.46, No.12, pp. 2701-2704, 2022, DOI:10.32604/biocell.2022.022229

Abstract Advances in regenerative medicine correlate strongly with progress in the use of adipose tissue-derived mesenchymal stem/stromal cells. The range of therapeutic indications has also expanded over recent years. Numerous recent studies have highlighted the primary importance of paracrine secretion by these cells. Though it is interesting to compare the different types of such secretions, we believe that exosomes (extra-cellular vesicles possessing the same properties as their source cells) will likely be the main key in tomorrow’s cell therapy. Exosomes also have many advantages compared to the direct use of cells, making these particles a major target in fundamental and translational… More >

KI-TAEK LIM^#,*, TEJAL V. PATIL^#, DINESH K. PATEL, SAYAN DEB DUTTA, KEYA GANGULY, AAYUSHI RANDHAWA

BIOCELL, Vol.46, No.10, pp. 2201-2208, 2022, DOI:10.32604/biocell.2022.020013

Abstract Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. Recently, MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment. Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. However, misconceptions and ethical issues still exist, such as MSCs being non-angiogenic, anti-apoptotic, and immunoregulatory competencies. Embryonic stem cells isolation primarily requires the consent of donors and can include… More >

ADRIANA L. FERREIRA, GUSTAVO C. PARIS, ALINE DE A. AZEVEDO, ERIKA A. C. CORTEZ, SIMONE N. CARVALHO, LAIS DE CARVALHO, ALESSANDRA A. THOLE^*

BIOCELL, Vol.46, No.8, pp. 1807-1813, 2022, DOI:10.32604/biocell.2022.019363

Abstract Mesenchymal stem cells (MSC) have pushed the field of stem cell-based therapies by inducing tissue regeneration, immunosuppression, and angiogenesis mainly through vesicles and soluble factors release (paracrine signaling). MSC-extracellular vesicles (MSC-EV) adaptable secretome and homing to injured sites allowed researchers to unlock a new era of cell-free based therapy. In parallel, nanoparticles (NP) have been explored in contributing to transport and drug delivery systems, giving drugs desired physical-chemical properties to exploit cell behavior. However, NPs can be quickly recognized by immune cells and cleared from circulation. In this viewpoint, we explore how combining both therapeutic strategies can improve efficacy and… More >

FUAD GANDHI TORIZAL^1,2,*, FRANSISCUS FIANO ANTHONY KERANS³, ANNISA KHUMAIRA¹

BIOCELL, Vol.46, No.8, pp. 1885-1891, 2022, DOI:10.32604/biocell.2022.019591

Abstract The potential of mesenchymal stem cells (MSCs) in regenerative medicine has been largely known due to their capability to induce tissue regeneration in vivo with minimum inflammation during implantation. This adult stem cell type exhibit unique features of tissue repair mechanism and immune modulation mediated by their secreted factors, called secretome. Recently, the utilization of secretome as a therapeutic agent provided new insight into cell-free therapy. Nevertheless, a sufficient amount of secretome is necessary to realize their applications for translational medicine which required a proper biomanufacturing process. Several factors related to their production need to be considered to produce a… More >

MASSIMO CONESE^1,*, AURELIO PORTINCASA²

BIOCELL, Vol.46, No.8, pp. 1815-1826, 2022, DOI:10.32604/biocell.2022.019448

Abstract The treatment of nonhealing and chronic cutaneous wounds still needs a clinical advancement to be effective. Both mesenchymal stem cells (MSCs), obtained from different sources, and their secretome derived thereof (especially exosomes) can activate signaling pathways related to promotion of cell migration, vascularization, collagen deposition, and inflammatory response demonstrating prohealing, angiogenetic and anti-scarring capacities. On the other hand, biodegradable biomimetic scaffolds can facilitate endogenous cell attachment and proliferation as well as extracellular matrix production. In this Review, we revise the complex composites made by biomimetic scaffolds, mainly hydrogels, and MSC-derived exosomes constructed for cutaneous wound healing. Studies demonstrate that there… More >

GIORDANO WOSGRAU CALLONI^1,*, MARCO AUGUSTO STIMAMIGLIO^2,*

BIOCELL, Vol.46, No.6, pp. 1375-1381, 2022, DOI:10.32604/biocell.2022.019681

Abstract Mesenchymal stem cells (MSCs) and their byproducts have been widely validated as potential therapeutic products for regenerative medicine. The therapeutic effects result mainly from the paracrine activity of MSCs, which consists of the secretion of bioactive molecules, whether dispersed in medium conditioned by cell culture or encapsulated in extracellular vesicles. The composition of the MSC secretome, which represents the set of these secreted cellular products, is crucial for the performance of the desired therapeutic functions. Different cell culture strategies have been employed to adjust the secretome composition of MSCs to obtain the best therapeutic responses for different clinical contexts. However,… More >

YUKA IKEDA, NOZOMI NAGASE, AI TSUJI, KURUMI TANIGUCHI, YASUKO KITAGISHI, SATORU MATSUDA^*

BIOCELL, Vol.46, No.5, pp. 1163-1167, 2022, DOI:10.32604/biocell.2022.018177

Abstract Stemness of cancer cells contains limitless self-renewal proliferation. For the purpose of proliferation, secretome might exert its effects via the paracrine signaling. Specific microRNAs enclosed in the secretome of cancer stem cells could regulate the expression of anti-proliferative APRO family proteins. The biological functions of APRO family proteins seems to be quite intricate, however, which might be a key modulator of microRNAs, then could regulate the proliferation of cancer cells. In addition to affecting proliferation/differentiation during cellular development, APRO family proteins might also play an imperious role on keeping homeostasis in healthy stem cells under a physiological condition. Therefore, relationship… More >

MICHELE DI COSOLA^1,§; STEFANIA CANTORE^2,§,*; MARIO GIOSUÈ BALZANELLI³; CIRO GARGIULO ISACCO^2,3; KIEU CAO DIEM NGUYEN^2,3; RAJIV SAINI²; ANNARITA MALCANGI⁴; MARGHERITA TUMEDEI⁵; MARIATERESA AMBROSINO¹; ANTONIO MANCINI²; SALVATORE SCACCO⁶; RICCARDO NOCINI⁷; LUIGI SANTACROCE²; ANDREA BALLINI^8,9,#,*; EDOARDO BRAUNER^10,#

BIOCELL, Vol.46, No.4, pp. 923-929, 2022, DOI:10.32604/biocell.2022.018409

Abstract The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs), so called dental-derived stem cells (D-dSCs) with self-renewing and multilineage differentiation capabilities. D-dSCs properties involves a strong paracrine component resulting from the high levels of bioactive molecules they secrete in response to the local microenvironment. Altogether, this viewpoint develops a general picture of current innovative strategies to employ D-dSCs combined with biomaterials and bioactive factors for regenerative medicine purposes, and offers information regarding the available scientific data and possible applications. More >

THAÍS CASAGRANDE PAIM, MÁRCIA ROSÂNGELA WINK^*

BIOCELL, Vol.46, No.4, pp. 913-922, 2022, DOI:10.32604/biocell.2022.018498

Abstract Mesenchymal stem cells (MSCs) play key roles in regenerative medicine by promoting tissue healing. MSCs can be isolated from different adult tissues and they are able to differentiate into several lineages. Due to their anti-inflammatory, angiogenic and immune-modulatory properties, MSCs are suitable for tissue engineering applications and, when associated with biomaterials, their benefits can be improved. Moreover, recently, MSCs have been studied for new clinical applications, such as in the treatment of patients with COVID-19. MSCs regenerative potential has been attributed to their secretome, which comprises extracellular matrix, soluble proteins and several elements, including the release of extracellular vesicles. Even… More >