NA HU1,2,#, SHIQING ZHANG2,3,#, AQUAN JIN2, LIANYING GUO2, ZHENYUN QU2, JUN WANG2,*
Oncology Research, Vol.30, No.2, pp. 89-97, 2022, DOI:10.32604/or.2022.027058
Abstract We aimed to explore the molecular mechanism that were involved in SPINK1-induced proliferation and clonogenic survival of human colorectal carcinoma (CRC) HT29 cells. Initially, we generated HT29 cells either permanently silencing or overexpressing SPINK1 protein. The results showed that SPINK1 overexpression (OE) significantly stimulated the proliferation and clonal formation of HT29 cells at the varied time points. Secondly, we found SPINK1 OE enhanced the ratio of LC3II/LC3I and the level of autophagy-related gene 5 (ATG5), whereas SPINK1 knockdown (Kd) reversed the above outcome under normal culturing and/or fasting condition in the cells, indicating its role in autophagy enhancement. Moreover, LC3-GFP-transfected… More >
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