OLIVIA A. ROBLES-RODRÍGUEZ1, MARÍA J. LOERA-ARIAS1,*, JOSÉ J. PÉREZ-TRUJILLO1, ARNULFO VILLANUEVA-OLIVO1, ERNESTO PICÓN-GALINDO1, LAURA VILLARREAL-MARTÍNEZ2, ADOLFO SOTO-DOMÍNGUEZ1, HUMBERTO RODRÍGUEZ-ROCHA1, ARACELY GARCÍA-GARCÍA1, ODILA SAUCEDO-CÁRDENAS1,3, ROBERTO MONTES DE OCA-LUNA1,*
BIOCELL, Vol.44, No.3, pp. 401-409, 2020, DOI:10.32604/biocell.2020.010064
- 22 September 2020
Abstract Human-induced pluripotent stem cells (iPSCs) are an accessible source of adult-derived, patient-specific
pluripotent stem cells for use in basic research, drug discovery, disease modeling, and stem cell therapy. Improving the
accessibility of methods to obtain iPSCs regardless of the cell source can enhance their clinical application. Therefore,
our purpose is to report a simple protocol to obtain iPS-like cells from urine-derived renal epithelial cells (RECs) using
different extracellular matrices and transfection reagents. In this study, we began by culturing urine-derived cells from
healthy donors to establish a primary culture of renal epithelial cells, followed by… More >
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