FEI KONG¹, QIYUAN TIAN², BINGLIN KUANG³, LILI SHANG⁴, XIAOXIAO ZHANG⁵, DONGYANG LI⁵, YING KONG^6,*

BIOCELL, Vol.48, No.4, pp. 665-675, 2024, DOI:10.32604/biocell.2024.046342 - 09 April 2024

Abstract Introduction: Myocardial ischemia-reperfusion (IR) injury has received widespread attention due to its damaging effects. Electroacupuncture (EA) pretreatment has preventive effects on myocardial IR injury. SLC26A4 is a Na+ independent anion reverse transporter and has not been reported in myocardial IR injury. Objectives: To find potential genes that may be regulated by EA and explore the role of this gene in myocardial IR injury. Methods: RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA pretreatment. Myocardial infarction size was detected by TTC staining. Serum CK,… More > Graphic Abstract

LONGSHENG FU¹, JINFANG HU¹, FENG SHAO², YAOQI WU¹, WEI BAI³, MINGJIN JIANG³, HAO CHEN⁴, LIHUA CHEN², YANNI LV^1,*

BIOCELL, Vol.47, No.8, pp. 1869-1883, 2023, DOI:10.32604/biocell.2023.028602 - 28 August 2023

Abstract Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation function of biochanin A on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion (MCAO) followed by 24 h reperfusion. The infarct volume of the brain was stained by TTC, while leakage of the brain was quantitatively stained by Evans blue, and the neurologic deficit score was measured. Microglial-induced morphologic changes were observed via immunofluorescence staining, and rolling and adhering leukocytes in… More >

JINFANG HU^1,#, JIANGEN AO^2,#, LONGSHENG FU^1,#, YAOQI WU¹, FENG SHAO³, TIANTIAN XU¹, MINGJIN JIANG⁴, SHAOFENG XIONG¹, YANNI LV^1,*

BIOCELL, Vol.47, No.7, pp. 1611-1622, 2023, DOI:10.32604/biocell.2023.028684 - 21 June 2023

Abstract Background: The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion (I/R) is little known. The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography. The molecular mechanism of D. odorifera leaves on cerebral I/R was investigated. Methods: Serial affinity chromatography based on D. odorifera leaves extract (DLE) affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h. Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed… More >

JING JIN^1,#, XINGHUA WANG^2,#, XIAOXIAO ZHENG³, JIAHUA LAN³, LI ZHENG³, YING CAI³, HUI CHEN⁴, HONGWEI WANG^5,*, LIFANG ZHENG^6,*

BIOCELL, Vol.47, No.3, pp. 619-626, 2023, DOI:10.32604/biocell.2023.026016 - 03 January 2023

Abstract Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury (CIRI). The exact mechanisms underlying I/R injury are unclear. In this study, we aimed to determine the role of m⁶A-modified methylase complex methyltransferase-like 3 (METTL3) in cerebral ischemia-reperfusion (I/R) injury. We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytes-cerebellar (MA-C) and the brain of middle cerebral artery occlusion (MCAO) model mice. METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation, which increased with METTL3 over-expression. Flow cytometry analysis showed that silencing METTL3 significantly enhanced More >

ZHENGUO SHI^#,*, QIAOYUN WU^#, HAIYAN SHI, SONGTIE YING, LIANG TAO

BIOCELL, Vol.46, No.4, pp. 979-988, 2022, DOI:10.32604/biocell.2022.015345 - 15 December 2021

Abstract NLRP3 inflammasome-mediated cell pyroptosis aggravates the development of cerebral ischemia/reperfusion (I/R) injury, and the aim of this study is to investigate the potential utilization of the Chinese medicine, Puerarin, in treating this disease. Through conducting in vitro and in vivo experiments, the present study illustrated that Puerarin regulated LncRNA double homeobox A pseudogene 8 (DUXAP8)/miR-223-3p axis to inactivate NLRP3-mediated pyroptotic cell death, resulting in the attenuation of I/R injury. Specifically, the cerebral I/R injury in rat models and hypoxia/reoxygenation (H/R) in primary hippocampus neuron (PHN) cells were inducted, which were subsequently exposed to Puerarin treatment. As expected,… More >

JIANRONG YANG^1,#, WAN CHEN^2,#, XING ZHOU^3,#, YAOXUAN LI^4,#, ZHIHUANG NONG⁵, LIYUAN ZHOU⁴, XUAN WEI⁴, XIAORONG PAN⁶, CHUNXIA CHEN⁷, WENSHENG LU³

BIOCELL, Vol.46, No.1, pp. 137-148, 2022, DOI:10.32604/biocell.2022.016807 - 28 September 2021

Abstract In this study, we investigated the protective effect of hyperbaric oxygen (HBO) on PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion and its possible mechanism. PC12 and H9C2 cell oxygen-glucose deprivation/reperfusion model were established. Cells were divided into a control group, model group, hyperbaric air (HBA) group and HBO group. The cell viability was detected by the CCK8 assay. Hoechst 33342 and PI staining assays and mitochondrial membrane potential (MMP) assays were used to detect cell apoptosis. The ultrastructure of cells, including autophagosomes, lysosomes, and apoptosis, were examined using a transmission electron microscope. The… More >

CHEN-LIANG TSAI¹, YU-HUEI LIN², CHIH-YING CHANGCHIEN³, CHIH-FENG CHIAN^1,#,*, CHI-HUEI CHIANG^4,#,*

BIOCELL, Vol.45, No.5, pp. 1201-1211, 2021, DOI:10.32604/biocell.2021.014279 - 12 July 2021

Abstract Primary lung graft dysfunction could significantly attribute to ischemia-reperfusion lung injury (IRLI) during transplantation surgery. β2-adrenergic agonists were one of the bronchodilators that had been well-established in the management of asthma and chronic obstructive pulmonary disease (COPD) with anti-inflammatory potency. By applying the model of isolated rat lung, we evaluated the efficacy of short-acting β2-agonist inhalation to ameliorate ischemia-reperfusion damage. The experiment protocol was 180 min of global ischemia and then reperfusion for 60 min. In the β2-agonist inhalation group, aerosolized albuterol was administrated prior ischemia procedure. Increased weight ratios of wet to dry lung… More >

ZHAOHUI HU^1,2, XIANGJUN DING³, YUYAO JI², XIAOHONG LIU^4,*, ZHIWEN DING^2,*

BIOCELL, Vol.45, No.3, pp. 745-749, 2021, DOI:10.32604/biocell.2021.013293 - 03 March 2021

Abstract Apurine/pyrimidine-free endonuclease 1 (APEX1) is a multifunctional enzyme that contributes to oxidizationmediated DNA-cleaved base excision repair and redox activation of transcription factors. However, the role of APEX1 during cardiomyocyte oxidative stress injury is not completely understood. In the present study, whether APEX1 protects oxidative damage-induced cardiomyocytes was investigated. mRNA and protein expression levels of APEX1 were downregulated in the mouse model of cardiac ischemia-reperfusion injury. Furthermore, the expression of APEX1 in hydrogen peroxide (H₂O₂)-treated neonatal mice cardiomyocytes was also decreased. APEX1 knockdown aggravated H₂O₂-treated cardiomyocyte apoptosis indexes. By contrast, APEX1 overexpression reversed H₂O₂-induced oxidative damage, as demonstrated More >

FEI TIAN^*, GANG LIU, LINLIN FAN, ZHONGYUN CHEN, YAN LIANG,

BIOCELL, Vol.45, No.1, pp. 49-56, 2021, DOI:10.32604/biocell.2021.013794 - 26 January 2021

Abstract Cerebral ischemia is due to the formation of blood clots or embolisms in the brain arteries, which leads to local brain tissue necrosis and neural cell apoptosis. Recent studies have shown that microRNA (miRNA) plays an important regulatory role in the pathological process of ischemic injury. The aim of this study is to investigate the role and the mechanism of miR-21-3p and drosophila mothers against decapentaplegic 2 (SMAD2) in cerebral ischemic reperfusion injured (CIRI) neural cells. The CIRI model was established by oxygen-glucose deprivation and recovery process for N2a cells. The cell viability and the… More >

QI SONG¹, JUN ZHANG¹, QIANG ZHANG¹, JING LIU¹, KE LV¹, JIALU YAO^1,2,3,*, YAFENG ZHOU^2,3,*

BIOCELL, Vol.44, No.4, pp. 623-629, 2020, DOI:10.32604/biocell.2020.012645 - 24 December 2020

Abstract Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by More >