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  • Open Access

    ARTICLE

    A New Micropropagation Technology of Tilia amurensis: In Vitro Micropropagation of Mature Zygotic Embryos and the Establishment of a Plant Regeneration System

    Shijie Lin1, Zimo Wang1, Hongbo Zhu2, Conghui Wang3, Hongfeng Wang2, Dawei Zhang1, Tianbing Gou1, Guangdao Bao1, Ye Luo1, Huaijiang He1, Zhonghui Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 277-289, 2024, DOI:10.32604/phyton.2024.046989

    Abstract Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture… More >

  • Open Access

    ARTICLE

    Advancing Wound Filling Extraction on 3D Faces: An Auto-Segmentation and Wound Face Regeneration Approach

    Duong Q. Nguyen1, Thinh D. Le3, Phuong D. Nguyen3, Nga T. K. Le2, H. Nguyen-Xuan3,*

    CMES-Computer Modeling in Engineering & Sciences, Vol.139, No.2, pp. 2197-2214, 2024, DOI:10.32604/cmes.2023.043992

    Abstract Facial wound segmentation plays a crucial role in preoperative planning and optimizing patient outcomes in various medical applications. In this paper, we propose an efficient approach for automating 3D facial wound segmentation using a two-stream graph convolutional network. Our method leverages the Cir3D-FaIR dataset and addresses the challenge of data imbalance through extensive experimentation with different loss functions. To achieve accurate segmentation, we conducted thorough experiments and selected a high-performing model from the trained models. The selected model demonstrates exceptional segmentation performance for complex 3D facial wounds. Furthermore, based on the segmentation model, we propose an improved approach for extracting… More > Graphic Abstract

    Advancing Wound Filling Extraction on 3D Faces: An Auto-Segmentation and Wound Face Regeneration Approach

  • Open Access

    ARTICLE

    Histological Assessment of Bone Regeneration in the Maxilla with Homologous Bone Graft: A Feasible Option for Maxillary Bone Reconstruction

    Sergio Henrique Gonçalves Motta1, Ana Paula Ramos Soares1, Juliana Campos Hasse Fernandes2, Gustavo Vicentis Oliveira Fernandes2,3,*

    Journal of Renewable Materials, Vol.12, No.1, pp. 131-148, 2024, DOI:10.32604/jrm.2023.043940

    Abstract Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ridges. Thus, the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB (lyophilized) graft and verify its reliability. Eight individuals were included from 2014 to 2018. The first surgery was performed to install homologous bone blocks in the maxilla. The period of the second intervention varied between 5 months and 15 days to 11 months (≈7.93 months). The biopsies were taken from the central region of the matured graft during the surgery for implant placement. All patients presented clinical and radiographic conditions… More >

  • Open Access

    ARTICLE

    Comparison of Apigenin, Quercetin and Kaempferol Accumulation and Total Flavonoid Content in Leaves, Embryogenic Cultures and Cell Suspension Cultures of Parsley (Petroselinum crispum)

    Laura Isabel Arias-Rodríguez1, Martha Alicia Rodríguez-Mendiola2,*, Carlos Arias-Castro2,*, Federico Antonio Gutiérrez Miceli1, Diana Reséndez Pérez3, María Celina Luján Hidalgo1, Juan José Villalobos Maldonado1, Norma Alejandra Mancilla Margalli2

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2807-2823, 2023, DOI:10.32604/phyton.2023.030396

    Abstract In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration. In our study, we specifically investigated the extract of Petroselinum crispum, a plant known for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly embryogenic cultures, and suspension… More >

  • Open Access

    REVIEW

    Adventitious Root Regeneration: Molecular Basis and Influencing Factors

    Lulu Zhi, Xiangyang Hu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2825-2840, 2023, DOI:10.32604/phyton.2023.030912

    Abstract Plant regeneration is a self-repair of the plant body in response to adverse conditions or damaged structures, and root regeneration allows the plant body to better adapt to its environment by supplementing the roots’ structure. Previous research has shown that adventitious roots can be made to occur from scratch in two ways. Studies that simulate adventitious root regeneration through natural conditions allow the regeneration process to be broadly divided into three stages: the perception of early signals, the massive accumulation of auxin, and the transformation of cell fate. The strength of regeneration, in turn, is influenced by wounding, stress, hormones,… More >

  • Open Access

    ARTICLE

    Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

    Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2489-2501, 2023, DOI:10.32604/phyton.2023.029492

    Abstract The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at higher concentrations where shooting rates… More >

  • Open Access

    ARTICLE

    Soil Moisture Rather than Soil Nutrient Regulates the Belowground Bud Bank of Rhizomatous Species Psammochloa villosa in Arid Sand Dunes

    Yawei Dong1, Ziyue Guo1, Qun Ma2, Zhiming Xin3, Jin Tao1, Jiatai Tian1, Jinlei Zhu3, Zhiming Zhang1,*, Jianqiang Qian1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1301-1309, 2023, DOI:10.32604/phyton.2023.027043

    Abstract In arid and semi-arid sand dune ecosystems, belowground bud bank plays an important role in population regeneration and vegetation restoration. However, the responses of belowground bud bank size and composition to sand burial and its induced changes in soil environmental factors have been rarely studied. In arid sand dunes of Northwestern China, we investigated belowground bud bank size and composition of the typical rhizomatous psammophyte Psammochloa villosa as well as three key soil environmental factors (soil moisture, total carbon and total nitrogen) under different depths of sand burial. Total buds and rhizome buds increased significantly with increasing burial depth, whereas… More >

  • Open Access

    REVIEW

    Understanding cell-extracellular matrix interactions for topology-guided tissue regeneration

    AAYUSHI RANDHAWA1,2, SAYAN DEB DUTTA1, KEYA GANGULY1, TEJAL V. PATIL1,2, RACHMI LUTHFIKASARI1, KI-TAEK LIM1,2,*

    BIOCELL, Vol.47, No.4, pp. 789-808, 2023, DOI:10.32604/biocell.2023.026217

    Abstract Tissues are made up of cells and the extracellular matrix (ECM) which surrounds them. These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life. This astonishing mechanical stress develops due to the interaction between the live cells and the non-living ECM. Cells in the matrix microenvironment can sense the signals and forces produced and initiate a signaling cascade that plays a crucial role in the body’s normal functioning and influences various properties of the native cells, including growth, proliferation, and differentiation. However, the matrix’s characteristic features also impact the repair and regeneration of the… More >

  • Open Access

    ARTICLE

    Tensile Properties and Wear Resistance of Mg Alloy Containing High Si as Implant Materials

    Mengqi Cong*, Yang Zhang, Yunlong Zhang, Xiao Liu, Yalin Lu, Xiaoping Li

    Journal of Renewable Materials, Vol.11, No.4, pp. 1977-1989, 2023, DOI:10.32604/jrm.2023.023849

    Abstract Magnesium alloy has been considered as one of the third-generation biomaterials for the regeneration and support of functional bone tissue. As a regeneration implant material with great potential applications, in-situ Mg2Si phase reinforced Mg-6Zn cast alloy was comprehensively studied and expected to possess excellent mechanical properties via the refining and modifying of Mg2Si reinforcements. The present study demonstrates that the primary and eutectic Mg2Si phase can be greatly modified by the yttrium (Y) addition. The size of the primary Mg2Si phases can be reduced to ~20 μm with an addition of 0.5 wt.% Y. This phenomenon is mainly attributed to… More > Graphic Abstract

    Tensile Properties and Wear Resistance of Mg Alloy Containing High Si as Implant Materials

  • Open Access

    REVIEW

    Dental pulp stem cells and banking of teeth as a lifesaving therapeutic vista

    SUKUMARAN ANIL1,2,*, RAMYA RAMADOSS3, NEBU G. THOMAS4, JASMIN M. GEORGE4, VISHNUPRIYA K. SWEETY4

    BIOCELL, Vol.47, No.1, pp. 71-80, 2023, DOI:10.32604/biocell.2023.024334

    Abstract Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest, process, and cryogenically preserve dental pulp stem cells. Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells. Teeth serve as a substantial source of mesenchymal stem cells, otherwise disposed of as medical waste. Care should be taken to store this treasure trove of stem cells. Collective responsibility of patients, dentists, and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the future. The dental pulp stem… More >

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