Fenglan Zhao^1,2,#, Shuwen Wu^1,#, Xue Meng¹, Jianping Xue^1,2, Yongbo Duan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1705-1716, 2024, DOI:10.32604/phyton.2024.052287 - 30 July 2024

Abstract Dōng líng căo, the dried aboveground parts of Isodon rubescens (Hemls.) Hara., is commonly consumed as a medicinal decoction or tea beverage. Natural beverages can be an important source of human dietary selenium (Se). However, how I. rubescens plants respond to exogenous Se remains unknown. In this study, a pot cultivation experiment was employed to investigate the phenotypic and physiological responses of I. rubescens plants exposed to Se. Fifteen days after applying different concentrations of sodium selenate to the soil, the Se enrichment capacity, growth indices, antioxidant capacities, and the content of flavonoids and diterpenoids were measured in… More >

SEHER YILMAZ^1,2,*, ZÜLEYHA DOĞANYIĞIT³, MERT OCAK⁴, EVRIM SUNA ARIKAN SÖYLEMEZ⁵, ASLI OKAN OFLAMAZ³, SÜMEYYE UÇAR⁶, ŞÜKRÜ ATE޹, AMMAD AHMAD FAROOQI⁷

Oncology Research, Vol.32, No.1, pp. 175-185, 2024, DOI:10.32604/or.2023.042350 - 15 November 2023

Abstract Melatonin is a versatile indolamine synthesized and secreted by the pineal gland in response to the photoperiodic information received by the retinohypothalamic signaling pathway. Melatonin has many benefits, such as organizing circadian rhythms and acting as a powerful hormone. We aimed to show the antitumor effects of melatonin in both in vivo and in vitro models through the mammalian target of rapamycin (mTOR) signaling pathway and the Argyrophilic Nucleolar Regulatory Region (AgNOR), using the Microcomputed Tomography (Micro CT). Ehrlich ascites carcinoma (EAC) cells were administered into the mice by subcutaneous injection. Animals with solid tumors were injected… More > Graphic Abstract

Zhipeng Sheng, Yuting Luan, Cong Xu, Jun Tao^*, Daqiu Zhao^*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 801-814, 2023, DOI:10.32604/phyton.2023.024953 - 29 November 2022

Abstract Herbaceous peony (Paeonia lactiflora Pall.), as a high-end cut flower in the international market, has high ornamental and medicinal values. But in Northern China, drought is a major environmental factor influencing the growth and development of P. lactiflora. Quantitative real-time polymerase chain reaction (qRT-PCR) can evaluate gene expression levels under different stress conditions, and stable internal reference is the key for qRT-PCR. At present, there is no systematic screening of internal reference for correcting gene expressions of P. lactiflora in response to drought stress. In this study, 10 candidate genes [ubiquitin (UBQ2), UBQ1, elongation factor 1-α (EF-1α), Histidine (His), eukaryotic… More >

Yuting Luan¹, Cong Xu¹, Xiaoxiao Wang¹, Daqiu Zhao¹, Jun Tao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1045-1059, 2022, DOI:10.32604/phyton.2022.018767 - 24 January 2022

Abstract The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed More >

Sicong Zheng^#, Jingjing Song^#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang^*, Qinglin Liu, Yuanzhi Pan

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 525-539, 2022, DOI:10.32604/phyton.2022.017629 - 26 October 2021

Abstract Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially… More >

Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259 - 22 April 2020

Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized data. Acer palmatum is an important colorful leaf ornamental tree species, and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been investigated. In this study, the expression stability of ten candidate reference genes, namely, Actin3, Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in two distinct tissues (leaves and roots) of A. palmatum under four different abiotic stress conditions (cold,… More >

G. P. CARDOZO¹ , E. V. SANTOS¹ , A. L. FACHIN¹, S. C. FRANÇA¹ , AND M. MARINS^1,2,*

BIOCELL, Vol.35, No.1, pp. 35-36, 2011, DOI:10.32604/biocell.2011.35.035

Abstract E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR. More >