Huan Shen^*1, Li Liu^†1, Yongjin Yang^*, Wenxing Xun^‡, Kewen Wei^‡, Guang Zeng^‡

Oncology Research, Vol.25, No.7, pp. 1141-1152, 2017, DOI:10.3727/096504017X14841698396784

Abstract Oral squamous cell carcinoma (OSCC) is a common cancer of the head and neck. Betulinic acid (BA) is a naturally occurring pentacyclic triterpenoid. The present study was designed to explore the effects of BA on OSCC KB cell proliferation in vitro and on implanted tumor growth in vivo and to examine the possible molecular mechanisms. The results showed that BA dose-dependently inhibited KB cell proliferation and decreased implanted tumor volume. In addition, BA significantly promoted mitochondrial apoptosis, as reflected by an increase in TUNEL+ cells and the activities of caspases 3 and 9, an increase… More >

Ayman I. Elkady^*†, Osama A. Abu-Zinadah^*, Rania Abd El Hamid Hussein^‡§

Oncology Research, Vol.25, No.6, pp. 897-912, 2017, DOI:10.3727/096504016X14816352324532

Abstract There is an urgent need to improve the clinical management of hepatocellular carcinoma (HCC), one of the most common causes of global cancer-related deaths. Zingibar officinale is a medicinal herb used throughout history for both culinary and medicinal purposes. It has antioxidant, anticarcinogenic, and free radical scavenging properties. Previously, we proved that the crude flavonoid extract of Z. officinale (CFEZO) inhibited growth and induced apoptosis in several cancer cell lines. However, the effect of the CFEZO on an HCC cell line has not yet been evaluated. In this study, we explored the anticancer activity of CFEZO against… More >

Zhihai Wang^*, Kui Liao^†, Wenqi Zuo^*, Xueliang Liu^*, Zhili Qiu^*, Zhitao Gong^*, Chuan Liu^*, Quan Zeng^*, Yi Qian^*, Liang Jiang^*, Youquan Bu^‡, Suling Hong^*, Guohua Hu^*

Oncology Research, Vol.25, No.1, pp. 123-136, 2017, DOI:10.3727/096504016X14732772150226

Abstract NFBD1, a signal amplifier of the p53 pathway, is vital for protecting cells from p53-mediated apoptosis and the early phase of DNA damage response under normal culture conditions. Here we investigated its expression in patients with nasopharyngeal carcinoma (NPC), and we describe the biological functions of the NFBD1 gene. We found that NFBD1 mRNA and protein were more highly expressed in NPC tissues than in nontumorous tissues. To investigate the function of NFBD1, we created NFBD1-depleted NPC cell lines that exhibited decreased cellular proliferation and colony formation, an increase in their rate of apoptosis, and More >

Yuanyuan Wang^*1, Qin Luo^*1, Xianlu He^†1, He Wei^*, Ting Wang^*, Jichun Shao^‡, Xinni Jiang^*

Oncology Research, Vol.26, No.6, pp. 889-899, 2018, DOI:10.3727/096504017X15009419625178

Abstract Recent studies revealed that emodin extracted from Chinese herbs exhibits an anticancer effect on different cancer types, including colon cancer. However, the mechanism is not well understood. In our study, we confirmed that emodin treatment inhibited cell viability and induced apoptosis in colon cancer cells. Further experiments found that emodin was also able to induce autophagy, which is indispensible for apoptosis induced by emodin. More interestingly, emodin treatment also results in mitochondrial dysfunction and ROS accumulation in colon cancer cells. Finally, we stressed that ROS accumulation is essential for autophagy and apoptosis induced by emodin. More >

Sanae Haga^*, Akira Kanno^†, Takeaki Ozawa^‡, Naoki Morita^§, Mami Asano^*¶,¶ and Michitaka Ozaki^*¶

Oncology Research, Vol.26, No.3, pp. 503-513, 2018, DOI:10.3727/096504017X15005102445191

Abstract Liver injury is often observed in various pathological conditions including posthepatectomy state and cancer chemotherapy. It occurs mainly as a consequence of the combined necrotic and apoptotic types of cell death. In order to study liver/hepatocyte injury by the necrotic type of cell death, we studied signal-regulated necrosis (necroptosis) by developing a new optic probe for detecting receptor-interacting protein kinase 1 (RIP)/RIP3 binding, an essential process for necroptosis induction. In the mouse hepatocyte cell line, TIB-73 cells, TNF-a/cycloheximide (T/C) induced RIP1/3 binding only when caspase activity was suppressed by the caspase-specific inhibitor z-VAD-fmk (zVAD). T/C/zVAD-induced… More >

Mahmuda Binte Monsur¹, Nasrin Akter Ivy¹, M. Moynul Haque², Mirza Hasanuzzaman³, Ayman EL Sabagh^4,5,*, Md. Motiar Rohman^6,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 497-517, 2020, DOI:10.32604/phyton.2020.09144

Abstract The research was conducted to investigate comparative oxidative damage including probable protective roles of antioxidant and glyoxalase systems in rice (Oryza sativa L.) seedlings under salinity stress. Seedlings of two rice genotypes: Pokkali (tolerant) and BRRI dhan28 (sensitive) were subjected to 8 dSm⁻¹ salinity stress for seven days in a hydroponic system. We observed significant variation between Pokkali and BRRI dhan28 in phenotypic, biochemical and molecular level under salinity stress. Carotenoid content, ion homeostasis, antioxidant enzymes, ascorbate and glutathione redox system and proline accumulation may help Pokkali to develop defense system during salinity stress. However, the activity More >

Atif Kamil¹, Mubarak Ali Khan¹, Muhammad AAsim², Nadir Zaman Khan², Raham Sher Khan¹, Muhsin Jamal³, Waqar Ahmad⁴, Mir Azam Khan⁴, Fazal Jalil⁴

BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and More >