Yong Zhao^2,#, Lili Li^1,#, Rui Luo¹, Mu Peng¹, Tongtong Jiang¹, Mingda Yin¹, Yanpeng Wen¹, Zihan Wang¹, Fenglan Huang^1,4,5,6,7,*, Fanjuan Meng^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.12, pp. 3237-3250, 2023, DOI:10.32604/phyton.2023.031495

Abstract Castor, scientifically known as Ricinus communis L., is among the top ten oil crops globally. It is considered a renewable resource and is commonly referred to as ‘green oil’. Castor seeds contain castor oil as their main component, which is predominantly composed of ricinoleic acid. This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor, resulting in the creation of mutant plants L1 and L2. The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129. The crude fat and ricinoleic acid levels of L1 and L2 were higher than those of NO.2129… More >

TINGTING HUANG^1,#, SHAN DENG^2,#, JIANGYUAN SHENG¹, DI ZHANG^1,*

BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314

Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK₃ type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE and RNAi… More >

YUQING HE¹, LI GUO¹, JUAN DING¹, HAOWEN LV¹, QUANRUI MA¹, CHEN LI¹, YU SHAO¹, QIANG LIU¹, CHUN ZHANG¹, HAO YANG^1,2,*, JUAN LIU^1,*

BIOCELL, Vol.45, No.2, pp. 417-426, 2021, DOI:10.32604/biocell.2021.013842

Abstract Schizophrenia is a common and serious mental illness characterized by severe impairments in thinking, emotions, and behaviors. Usually, the cognitive deficits of schizophrenia are closely associated with abnormal neurogenesis due to the hypofunction of certain neural receptors such as N-methyl-D-aspartate receptors (NMDARs), which mediates neurotransmission. However, little is known about the involvement of NMDAR1 in regulating hippocampal neurogenesis in schizophrenia. In the current study, we present evidence suggesting that NMDAR1 regulates hippocampal neurogenesis as lentivirus-mediated shRNA silencing NMDAR1 gene or blocking with MK-801 results in abnormal neurogenesis consistently found in schizophrenia. The important finding was clearly demonstrated by the multiparametric… More >