TIEJUN ZHAO, RIQIANG DENG^*, MENGQIU CHEN, XUNZHANG WANG

BIOCELL, Vol.46, No.7, pp. 1675-1686, 2022, DOI:10.32604/biocell.2022.018166

Abstract Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism. In this study, through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection, 3463 pieces of time-series differentially expressed RNA (1,200 mRNA and 2,263 lncRNA) are identified and justified by experimental verification of randomly selected samples from them, proving the validity of the bioinformatical analysis on this topic. Functional enrichment analysis and target prediction are performed on those differentially expressed… More >

LIMIN CHEN^1,2, XIAOWEI LI¹, TIANJUN HE², QUANCONG WU², TINGTING CHEN³, JUN HUANG^1,*, YAOBIN LU^1,*

BIOCELL, Vol.46, No.3, pp. 821-828, 2022, DOI:10.32604/biocell.2021.016450

Abstract Venom plays several important roles in the life of the fire ant, Solenopsis invicta Buren. Laboratory rearing significantly affected the toxin component of S. invicta. However, the molecular mechanism of the change of venomous components when kept in the laboratory is unclear. In this study, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in the venom gland of S. invicta at 0, 10, and 60 days after laboratory rearing. The RNA-Seq results showed that the expression of a large number of genes changed. The DEGs were involved in multiple pathways, including proteolysis, serine-type endopeptidase, and allergen. Furthermore,… More >

SAMUELE GRECO^1,#, ELISA D’AGOSTINO^2,#, CHIARA MANFRIN¹, ANASTASIA SERENA GAETANO¹, GAEL FURLANIS¹, FRANCESCA CAPANNI¹, GIANFRANCO SANTOVITO², PAOLO EDOMI¹, PIERO GIULIO GIULIANINI¹, MARCO GERDOL^1,*

BIOCELL, Vol.45, No.6, pp. 1611-1619, 2021, DOI:10.32604/biocell.2021.016121

Abstract We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic cephalopods, which compensates for the decreased ability… More >

JOE R. DELANEY^*

BIOCELL, Vol.45, No.5, pp. 1167-1170, 2021, DOI:10.32604/biocell.2021.017296

Abstract Single-cell sequencing data has transformed the understanding of biological heterogeneity. While many flavors of single-cell sequencing have been developed, single-cell RNA sequencing (scRNA-seq) is currently the most prolific form in published literature. Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. One biologically relevant variable is readily inferred from scRNA-seq gene count tables regardless of individual gene representation within single cells: aneuploidy. Since hundreds of genes are present on chromosome arms, high-quality inferences of aneuploidy can be made… More >

Qi Zhang^1,#, Pengtao Li^2,#, Aiying Liu¹, Shaoqi Li¹, Quanwei Lu², Qun Ge¹, Junwen Li¹, Wankui Gong¹, Xiaoying Deng¹, Haihong Shang^1,3, Yuzhen Shi^1,*, Youlu Yuan^1,3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 837-858, 2021, DOI:10.32604/phyton.2021.014437

Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC₅F_3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC₇F₂ with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including two parents… More >

TIANXIANG ZHANG^1,#, MEISHAN ZHANG^1,#, MINGHUI SHI^1,#, WEIJIANG JIN^1,#, SHUMIAO ZHANG^2,#, MENGYUAN FAN³, YIMENG LI¹, YUPING MENG², XUELIN TIAN⁴, SHUQIANG LIU^1,*, DEFU HU^1,*

BIOCELL, Vol.45, No.2, pp. 281-306, 2021, DOI:10.32604/biocell.2021.010277

Abstract Male muskrats (Ondatra zibethicus L.) secrete musk from their scent glands during musk secretion season. Musk plays an important role as a communication pheromone during the breeding season. In this study, gas chromatography–mass spectrometry (GC–MS) was used to analyze the main components of musk. The GC–MS results after methyl esterification showed that 71.55% of the musk is composed of fatty acids. The other components of muskrat musk include cholesterol (9.31%) and other organics. Transcriptome comparison between musk secretion and non-secretion seasons showed significant changes in the scent glands for 53 genes involved in fatty acid and cholesterol synthesis and metabolism… More >

XIANLIANG HOU^1,2,3,#, DONGE TANG^1,#, FENGPING ZHENG^1,#, MINGLIN OU³, YONG XU¹, HUIXUAN XU¹, XIAOPING HONG⁴, XINZHOU ZHANG¹, WEIER DAI⁵, DONGZHOU LIU^4,*, YONG DAI^1,*

BIOCELL, Vol.44, No.4, pp. 559-582, 2020, DOI:10.32604/biocell.2020.011022

Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by abnormal cellular and humoral immune responses and excessive autoantibody production. The precise pathologic mechanism of SLE remains elusive. The advent of single-cell RNA sequencing (scRNA-seq) enables unbiased analysis of the molecular differences of cell populations at the single-cell level. We used scRNA-seq to profile the transcriptomes of peripheral blood mononuclear cells from an SLE patient compared with a healthy control (HC). A total of 16,021 cells were analyzed and partitioned into 12 distinct clusters. The marker genes of each cluster and the four major immune cell types (B cells,… More >

Pengtao Li^1,2,#,*, Quanwei Lu^1,#, Xianghui Xiao³, Rui Yang³, Xixi Duan¹

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 129-144, 2021, DOI:10.32604/phyton.2021.012928

Abstract Fiber length is a critical trait that principally determines cotton spinning quality, while Upland cotton as the most widely cultivated Gossypium species around the world subjects to the relatively ordinary fiber performance. Chromosome segment substitution lines (CSSLs) have been introduced in cotton breeding to take full advantages of superior fiber quality and high yield from Sea Island and Upland cotton, respectively, which serve as ideal materials for elucidating the genetic mechanism of complex quantitative traits. Here, three CSSLs derived from CCRI45 (G. hirsutum) × Hai1 (G. barbadense), two superior (MBI7561 and MBI7747) and one (MBI7285) with ordinary fiber-quality, were subjected… More >

Chongchong Yan¹, Qianqian Wang¹, Nan Zhang², Jiajia Wang¹, Xuexiang Ren¹, Bingjie Xue³, Xiaojing Pu⁴, Zhihong Xu⁴, Huajun Liao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 561-586, 2020, DOI:10.32604/phyton.2020.010322

Abstract Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity… More >

Jingsen Liu^1,2, Jinqi Ma^1,2, Ai Lin^1,2, Chao Zhang^1,2, Bo Yang^1,2, Liyuan Zhang^1,2, Lin Huang^1,2, Jiana Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 253-273, 2020, DOI:10.32604/phyton.2020.09334

Abstract Invertase (INV), a key enzyme in sucrose metabolism, irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, thus playing important roles in plant growth, development, and biotic and abiotic stress responses. In this study, we identified 27 members of the BnaINV family in Brassica napus. We constructed a phylogenetic tree of the family and predicted the gene structures, conserved motifs, cis-acting elements in promoters, physicochemical properties of encoded proteins, and chromosomal distribution of the BnaINVs. We also analyzed the expression of the BnaINVs in different tissues and developmental stages in the B. napus cultivar Zhongshuang 11 using qRT-PCR. In… More >