Anastasia Vylegzhanina^1,2, Irina Shalaginova^2,*, Dana Korolevich¹, Dmitry Katserov¹, Alexandra Semenova¹, Maria Sidorova¹, Sergey Eresko³, Marat Airapetov³, Marina Pavlova², Anna Levina², Natalia Dyuzhikova²

BIOCELL, Vol.49, No.10, pp. 2007-2031, 2025, DOI:10.32604/biocell.2025.071198 - 22 October 2025

Abstract Objectives: Chronic stress can trigger neuroinflammation and gut microbiota alterations, contributing to post-stress disorders. Individual differences in stress responses, shaped by genetic and physiological factors, require better characterization. We aimed to investigate the long-term effects of chronic stress in rats selectively bred for high and low nervous system excitability. Methods: Adult male rats from two strains selectively bred for high (HT) and low (LT) excitability thresholds of the nervous system underwent a 15-day chronic emotional-pain stress protocol. Behavioral assessments (elevated plus maze), cytokine levels (TNF, IL-1β, IL-6, IL-10) in the hippocampus and amygdala measured by… More >

Ying Wang¹, Weijun Zhang¹, Fei Cai¹, Yong Tao^2,*

Congenital Heart Disease, Vol.19, No.6, pp. 653-669, 2024, DOI:10.32604/chd.2025.060406 - 27 January 2025

Abstract Background: Previous studies combined integrated scRNA-seq with bulk RNA data to screen biomarkers for cardiomyopathy. This study extended this approach to identify biomarkers specific for hypertrophic cardiomyopathy (HCM). Methods: Datasets GSE36961, GSE130036, GSE249925 and GSE203274 were analyzed in this study. ScRNA-seq analysis was employed to identify distinct cell populations. Differentially expression analysis was conducted to screen vascular endothelial cells (VECs)-related feature genes. After calculating VECs score, WGCNA was used to correlate gene modules with the VECs score. Key HCM biomarkers were determined using random forest analysis, and LASSO regression analyses to construct a diagnostic model… More >

JI SHI^1,2, YE ZHANG², YI CHEN², TANGJUN GUO^3,*, HAOZHE PIAO^2,*

BIOCELL, Vol.48, No.8, pp. 1241-1263, 2024, DOI:10.32604/biocell.2024.051049 - 02 August 2024

Abstract Background: Glioblastoma multiforme (GBM) is the most general malignancy of the primary central nervous system that is characterized by high aggressiveness and lethality. Transmembrane protein 159 (TMEM159) is an endoplasmic reticulum protein that can form oligomers with seipin. The TMEM159-seipin complex decides the site of lipid droplet (LD) formation, and the formation of LDs is a marker of GBM. However, the role of TMEM159 in the progression of GBM has not been investigated to date. Methods: In this study, we examined the genes that may be associated with patient prognosis in GBM by bioinformatics analyses,… More >

JIAOE SHENG¹, ZUMIN YI², SANSHAN HE¹, QINGCHAO WU¹, XIA HUANG¹, GUOQING YAN¹, YUFANG DAI^1,*, LINCHONG SU^1,*

BIOCELL, Vol.48, No.7, pp. 1095-1104, 2024, DOI:10.32604/biocell.2024.028421 - 03 July 2024

Abstract Introduction: Cholic acid (CA) is a natural steroid useful in treating chronic bronchitis and cholecystitis. On the other hand, its potential impact on osteoarthritis (OA) is unknown. Objective: Using an in vitro and in vivo osteoarthritis model, we sought to assess the chondroprotective properties of CA. Methods: We employed the Cell Counting Kit-8 to measure the impact of CA on chondrocyte activity to assess the toxicity of the cells. Multiple molecular biology experimental techniques were used to investigate potential signaling pathways that CA may use to prevent inflammation and give chondrocytes protection. Furthermore, how CA affects the OA… More >

Xiaozhuo Xu^1,2, Yilin Huang^1,2, Xu Han^2,*

Congenital Heart Disease, Vol.19, No.2, pp. 233-246, 2024, DOI:10.32604/chd.2024.050231 - 16 May 2024

Abstract Background: Hypoplastic left heart syndrome (HLHS) is one of the most challenging congenital heart diseases in clinical treatment. In cardiac tissues, resident macrophages fulfill critical functions in maintaining a stable cardiac state and have strong regenerative capacity and organ specificity. However, the molecular mechanisms of macrophages in HLHS remained unclear. Methods: Single-nucleus RNA sequencing (snRNA-seq) data of HLHS and healthy control (donors) samples obtained from the Gene Expression Omnibus (GEO) database were normalized and clustered using the Seurat package. The “FindMarkers” function was used to screen differentially expressed genes (DEGs) between the HLHS and donor… More > Graphic Abstract

GERWIN HELLER^1,*, THORSTEN FUEREDER¹, ALEXANDER MICHAEL GRANDITS¹, ROTRAUD WIESER^1,2,*

Oncology Research, Vol.32, No.1, pp. 1-17, 2024, DOI:10.32604/or.2023.044774 - 15 November 2023

Abstract Head and neck squamous cell carcinoma (HNSCC) is one of the most frequent cancers worldwide. The main risk factors are consumption of tobacco products and alcohol, as well as infection with human papilloma virus. Approved therapeutic options comprise surgery, radiation, chemotherapy, targeted therapy through epidermal growth factor receptor inhibition, and immunotherapy, but outcome has remained unsatisfactory due to recurrence rates of ~50% and the frequent occurrence of second primaries. The availability of the human genome sequence at the beginning of the millennium heralded the omics era, in which rapid technological progress has advanced our knowledge… More >

Aisa Zulibiya^1,2,#, Jing Wen^3,#, Huiqing Yu^3,#, Xiaoming Chen³, Lei Xu³, Xiao Ma^1,2, Baojian Zhang^1,2,*

Congenital Heart Disease, Vol.18, No.6, pp. 611-625, 2023, DOI:10.32604/chd.2023.047689 - 19 January 2024

Abstract Background: Tetralogy of Fallot (TOF) is a very common cyanotic congenital heart disease. Endothelial-to-mesenchymal transition (EndoMT) is recognized as a physiological mechanism involved in embryonic heart development and endothelial formation. However, there is still a gap in the reports related to the mechanism of EndoMT development in TOF. Methods: First, transcriptomic data of single cell nuclei of TOF and Donor were obtained based on the Gene Expression Omnibus (GEO) database, and the data were normalized and clustered by dimensionality reduction using the Seurat package. Subsequently, differentially expressed genes (DEGs) between TOF and Donor were screened… More >

Yanyan Pu^1,#, Liwen Wang^2,#, Leilei Li³, Yujun Si⁴, Shubin Xie², Yunzhe Cong¹, Dong Wang¹, Yongchao Gong¹, Rumei Tian¹, Xue Chen³, Xiaoyan Zhang¹, Min Liu¹, Hanfeng Ding^1,3,*, Nana Li^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822 - 11 April 2023

Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total… More >

TINGTING SUN, XUE SUN, XIN WANG, RUI GUO, YUANHUA YU^*, LE GAO^*

BIOCELL, Vol.47, No.4, pp. 879-889, 2023, DOI:10.32604/biocell.2023.026229 - 08 March 2023

Abstract Background: Aldo-keto oxidoreductase (AKR) inhibitors could reverse the resistance of several cancer cells to cis-platin, but their role in resistance remains unclear.Methods: We verified the difference of AKR1Cs expression by Western blot, RNA sequencing and qRT-PCR. The differences of AKR1Cs expression were analyzed and inferred. Use Assay of NADH and NAD⁺ content to verify the inference. The Docking experience was used to verify the affinity between MPA, MCFLA, MLS and AKR1C3.Results: Our RNA-seq results showed de novo NAD biosynthesis-related genes and NAD(P)H-dependent oxidoreductases were significantly upregulated in cis-platin-resistant HepG2 hepatic cancer cells (HepG2-RC cells) compared with HepG2 cells.… More >

Lina Tang^1,2, Haiyan Hu², Yan Zhou², Yujing Huang², Yonggang Wang², Yawen Zhang², Jinrong Liang², Zhenxin Wang^1,*

Oncologie, Vol.24, No.4, pp. 913-925, 2022, DOI:10.32604/oncologie.2022.026296 - 31 December 2022

Abstract Objective: To investigate the expression of alpha–smooth muscle actin (ACTA2) in osteosarcoma tissues and its relationship with prognosis. Methods: Prognostic analysis of lung metastasis–related genes in osteosarcoma using the TCGA database. Single-cell sequencing detected the expression of ACTA2 in 11 osteosarcoma tissues. Paraf- fin-embedded tissues of 74 osteosarcoma patients treated at the Sixth People’s Hospital of Shanghai Jiao Tong University from 2014 to 2019 were collected, and tissue microarrays were prepared. ACTA2 expression was detected and scored by immunohistochemistry. According to the median value of the ACTA2 histochemical score, 74 patients were divided into two groups, the… More >