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Search Results (11)
  • Open Access

    ARTICLE

    Linkage Mapping and QTL Analysis of Isoflavones Composition in Soybean Seeds

    Songnan Yang1, Miao Zhang1, Rongrong Yao1, Liangyu Chen1, Weixuan Cong1, Dan Yao2, Jian Zhang1,3, Jun Zhang1,*, Xueying Li1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2209-2225, 2024, DOI:10.32604/phyton.2024.055046 - 30 September 2024

    Abstract The high isoflavones content of soybeans is an important breeding goal due to the demonstrated benefits of isoflavones to human health and their association with plant resistance. In this study, quantitative trait loci (QTL) mapping for soybean isoflavone aglycones, including daidzin, glycerin, and genistin, and total isoflavones content was performed in a population of 178 F2:6 recombinant inbred lines (RILs) which was generated from a cross between varieties Jinong 17 and Jinong 18. A genetic linkage map covering 1248 cM was constructed using the simple sequence repeat (SSR) molecular markers. The results revealed 22 isoflavone-related QTLs,… More >

  • Open Access

    ARTICLE

    Combining QTL Mapping and Multi-Omics Identify Candidate Genes for Nutritional Quality Traits during Grain Filling Stage in Maize

    Pengcheng Li1,2,#, Tianze Zhu1,#, Yunyun Wang1,2, Shuangyi Yin1, Xinjie Zhu1, Minggang Ji1, Wenye Rui1, Houmiao Wang1, Zefeng Yang1,2,*, Chenwu Xu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1441-1453, 2024, DOI:10.32604/phyton.2024.052219 - 30 July 2024

    Abstract The nutritional composition and overall quality of maize kernels are largely determined by the key chemical components: protein, oil, and starch. Nevertheless, the genetic basis underlying these nutritional quality traits during grain filling remains poorly understood. In this study, the concentrations of protein, oil, and starch were studied in 204 recombinant inbred lines resulting from a cross between DH1M and T877 at four different stages post-pollination. All the traits exhibited considerable phenotypic variation. During the grain-filling stage, the levels of protein and starch content generally increased, whereas oil content decreased, with significant changes observed between… More >

  • Open Access

    ARTICLE

    Quantitative Trait Loci (QTL) Mapping and Marker Analysis of Fatty Acids in Peanut

    Xiao Han, Songnan Yang, Xueying Li, Qiulin Wu, Yongyi Xing, Jun Zhang*, Fenglou Ling*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2577-2589, 2023, DOI:10.32604/phyton.2023.029440 - 28 July 2023

    Abstract Peanut, with high oil content, has been a major oil and food crop globally. The compositions of the fatty acids are the common factors in determining the oil quality. In the present study, an F2 segregated population with 140 individuals derived from the cross of Weihua8 (a cultivar) and 12L49 (a line with high oleic acid concentration) was used to construct a genetic map and conduct QTL mapping analysis. A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci. Within the 103 SSR loci, a genetic… More >

  • Open Access

    ARTICLE

    Introgression of Drought Tolerance into Elite Basmati Rice Variety through Marker-Assisted Backcrossing

    Muhammad Sabar1,2,#, Shahzad Amir Naveed1,3,4,#,*, Shahid Masood Shah5, Abdul Rehman Khan5, Muhammad Musaddiq Shah6, Tahir Awan1, Muhammad Ramzan Khan3, Zaheer Abbas3, Muhammad Arif1,7,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1421-1438, 2023, DOI:10.32604/phyton.2023.025801 - 09 March 2023

    Abstract Drought is one of the major abiotic threat to rice production in the context of climate change. Super Basmati is an elite, fine grain basmati rice variety grown in Punjab, Pakistan. Due to drought sensitive in nature, its yield has been facing an alarming situation in production because of gradual decrease in irrigated water for a couple of years. Three reported novel QTLs for drought tolerance were selected for incorporation into Super Basmati by employing marker assisted selection strategy. IR55419-04 with novel QTLs was used as a donor parent. Foreground selection was performed by applying… More >

  • Open Access

    ARTICLE

    Identification of QTLs for Yield and Associated Traits in F2 Population of Rice

    Rohini Bhat1,*, Anil Kumar Singh2, Muntazir Mushtaq3, Romesh Kumar Salgotra2, Manmohan Sharma2, Basharat Ahmad Bhat4, Umer Basu5, Ibrahim Al-Ashkar 6, Mohammad Anwar Hossain7,*, Akihiro Ueda8, Ayman El Sabagh9

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2439-2459, 2022, DOI:10.32604/phyton.2022.020100 - 12 July 2022

    Abstract Identification of quantitative trait loci (QTLs) controlling yield and yield-related traits in rice was performed in the F2 mapping population derived from parental rice genotypes DHMAS and K343. A total of 30 QTLs governing nine different traits were identified using the composite interval mapping (CIM) method. Four QTLs were mapped for number of tillers per plant on chromosomes 1 (2 QTLs), 2 and 3; three QTLs for panicle number per plant on chromosomes 1 (2 QTLs) and 3; four QTLs for plant height on chromosomes 2, 4, 5 and 6; one QTL for spikelet density on… More >

  • Open Access

    ARTICLE

    Discovering Candidate Chromosomal Regions Linked to Kernel Size-Related Traits via QTL Mapping and Bulked Sample Analysis in Maize

    Hameed Gul1, Mengya Qian1, Mohammad G. Arabzai1,2, Tianhui Huang1, Qiannan Ma1, Fangyu Xing1, Wan Cao1, Tingting Liu1, Hong Duan1, Qianlin Xiao1,*, Zhizhai Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1429-1443, 2022, DOI:10.32604/phyton.2022.019842 - 14 March 2022

    Abstract Kernel size-related traits, including kernel length, kernel width, and kernel thickness, are critical components in determining yield and kernel quality in maize (Zea mays L.). Dissecting the phenotypic characteristics of these traits, and discovering the candidate chromosomal regions for these traits, are of potential importance for maize yield and quality improvement. In this study, a total of 139 F2:3 family lines derived from EHel and B73, a distinct line with extremely low ear height (EHel), was used for phenotyping and QTL mapping of three kernel size-related traits, including 10-kernel length (KL), 10-kernel width (KWid), and 10-kernel… More >

  • Open Access

    ARTICLE

    Meta-Analysis of Flowering-Related Traits and Mining of Candidate Genes in Maize

    Jianyu Lu1, Qingping Jiang1, Tongyu Liu1, Yangyang Zhou1, Mengtong Liu1, Jing Qu2, Yiyong Ma2,*, Shuyan Guan2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1199-1209, 2022, DOI:10.32604/phyton.2022.019298 - 14 February 2022

    Abstract Maize flowering is an important agronomic character, which is controlled by quantitative trait loci (QTL). Over the years, a large number of flowering-related QTL have been found in maize and exist in public databases. However, combining these data, re-analyzing and mining candidate loci and fine mapping of flowering-related traits to reduce confidence intervals has become a hot issue in maize. In this study, the QTL of 6 important agronomic traits of maize flowering were collected from 15 published articles, including flowering period (DA), Days to tasseling (DTT), Days to silking (DS), Days to pollen shedding More >

  • Open Access

    ARTICLE

    Fine Mapping and Candidate Gene Prediction of the Quantitative Trait Locus qPL8 for Panicle Length in Rice

    Lin Zhang1,2,*, Yiting Zou1, Zhong Bian1, Dong Xie1, Hadi Yeilaghi1, Xiaolei Fan1,2, Dongsheng Zhao1,2, Qiaoquan Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 789-802, 2021, DOI:10.32604/phyton.2021.014880 - 30 March 2021

    Abstract Rice panicle is the sink organ where assimilation product accumulates, and its morphology determines the rice yield. Panicle length has been suggested as a yield-related trait, but the genetic factor for its control is still limited. In this study, we carried out fine-mapping of qPL8, a QTL identified for panicle length in our previous work. Near isogenic line (NIL) with qPL8 exhibited elongated panicle without obvious effect on other panicle elements. With five key recombinants from NIL population, the locus was finally narrowed down to a 278-kb region, where 44 genes are annotated. By comparing the More >

  • Open Access

    ARTICLE

    Grapevine Phenological Quantitative Trait SSR Genotyping Using High-Throughput HRM-PCR Analysis

    Murad Awad1, Photini V. Mylona2, Alexios N. Polidoros1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 905-923, 2020, DOI:10.32604/phyton.2020.011235 - 09 November 2020

    Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The… More >

  • Open Access

    ARTICLE

    Quantitative trait loci associated with water deficit tolerance in Arabidopsis thaliana (L.) Heynh.

    Gutiérrez-Díez A1, GE Salinas-García1, L Iracheta-Donjuan2, JA Torres-Castillo1, N Mayek-Pérez3

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 203-208, 2013, DOI:10.32604/phyton.2013.82.203

    Abstract Gene and quantitative trait loci (QTLs) localization in a model species allow to know their organization in the genome, and the possibility for the isolation and cloning of genes with agronomic importance. Seventy-three recombinant inbred lines (RILs) derived from the cross of Arabidopsis thaliana (L.) Heynh. ecotypes Columbia (Col) x Landsberg erecta (Ler) were grown in a greenhouse under contrasting soil moisture conditions (irrigated and water deficits). During their growth, some phenological (days to flowering) and plant growth traits (plant height, and number of nodes and leaves per plant) were measured. Significant differences for plant height More >

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