MIN LIU^1,*, XIA LI², JUN LIU¹, YU LIU¹

BIOCELL, Vol.48, No.10, pp. 1475-1487, 2024, DOI:10.32604/biocell.2024.053227 - 02 October 2024

Abstract Background: Alveolar macrophage pyroptosis exacerbates inflammatory lung diseases, and tanshinone IIA is known for its anti-inflammatory properties. Thus, understanding how tanshinone IIA affects alveolar macrophage pyroptosis is essential. Methods: NR8383 cells were exposed to lipopolysaccharide (LPS) and adenosine triphosphate (ATP). We assessed cell viability, pyroptosis, and the expression of triggering receptors expressed on myeloid cells 2 (TREM2), p-β-catenin, β-catenin, and pyroptosis-related factors. We also examined the interaction between tanshinone IIA and TREM2. Results: Co-stimulation with LPS and ATP significantly reduced NR8383 cell viability, increased pyroptosis, and upregulated pyroptosis-associated factors. Treatment with tanshinone IIA mitigated these effects.… More >

YU TANG¹, WENXIAO HU^2,*, YAJUN PENG¹, XIANGDONG LING²

BIOCELL, Vol.48, No.10, pp. 1503-1516, 2024, DOI:10.32604/biocell.2024.052692 - 02 October 2024

Abstract Background: Podocyte injury is crucial in diabetic kidney disease (DKD) progression, and the mechanism remains unclear. The previous studies indicated Diosgenin played a key role in inhibiting podocyte injury progression. However, more research is needed to explore Diosgenin in inhibiting-molecular mechanisms in the process of podocyte injury. Methods: The content of Diosgenin in HeShenwan was detected by High-Performance Liquid Chromatography-mass spectrometry (HPLC-MS) method. The podocyte injury model was constructed by high glucose (HG)-induced mpc5 cells. The Cell Counting Kit-8 (CCK-8) assay was utilized to evaluate the activity of mpc5 cells. Pyroptosis in mpc5 cells was… More > Graphic Abstract

LINPEI WANG^1,2, JIAWEI WANG², CHUNFENG SHI², WEI WANG^2,*, JIAN WU^1,*

BIOCELL, Vol.48, No.9, pp. 1299-1310, 2024, DOI:10.32604/biocell.2024.051756 - 04 September 2024

Abstract Background: Alpha/beta hydrolase domain-containing protein 17C (ABHD17C) is a depalmitoylation enzyme that removes the S-palmitoylation of targeted proteins. The hepatocellular carcinoma (HCC) cells SNU449 and Hep3B use ABHD17C as an oncogene; however, the exact mechanism of this action is yet unknown. Methods: The expression of ABHD17C in liver cancer tissues was analyzed by bioinformatics, and the expression of ABHD17C in clinical liver cancer tissues and adjacent normal tissues was detected. Then, the proliferative viability of HCC cells after overexpression or knockdown of ABHD17C was examined, and pyroptosis and apoptosis proteins were detected. Results: ABHD17C was overexpressed in More >

JUNYING XU^1,#, PING LI^1,#, GE WANG², DAQIANG YE^1,*, XIUFU TANG^3,*

BIOCELL, Vol.48, No.2, pp. 283-291, 2024, DOI:10.32604/biocell.2023.045429 - 23 February 2024

Abstract Background: α7 nicotinic acetylcholine receptor (α7nAChR) has been demonstrated to be involved in numerous of inflammatory diseases. Cell pyroptosis is a kind of cell death accompanied by inflammation. Objectives: The objective of this work is to explore the function of α7nAChR on cell pyroptosis in cervical cancer cells. Methods: Immunoblotting, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay were employed to examine the function of α7nAChR on cell pyroptosis and metabolic changes. Results: Herein, we found that α7nAChR inhibition led to cell pyroptosis in HeLa and SiHa of cervical cancer cells, which was attributed to the… More >

WEI ZHENG¹, CHENG LIU^2,*

BIOCELL, Vol.48, No.2, pp. 293-301, 2024, DOI:10.32604/biocell.2023.044211 - 23 February 2024

Abstract Objective: Intracranial aneurysm (IA) represents a devastating disease with high rates of disability and mortality, which is initiated by dysfunction of endothelial cells (ECs). Evidence suggests the dysregulation of the E3 ubiquitin ligase family during EC injury. In this work, the role of an E3 ubiquitin ligase, casitas B lymphoma-B (CBL-B), was explored in human brain microvascular EC (HBMEC) function through the NLRP3 pathway. Methods: In vitro IA model was induced by treating HBMECs with oxidized low-density lipoprotein (ox-LDL). The levels of CBL-B and pyroptosis-related proteins NLRP3, ASC, cleaved caspase-1, and GSDME-N were determined by real-time-quantitative… More >

LIPING YE^1,2,3,#, HUIYAN SUN^4,#, XINHUA LIANG^2,#, WENXU PAN², LI XIANG^2,3, WENJUN DU², LANLAN GENG², WANFU XU^2,3,*, SITANG GONG^1,2,3,*

BIOCELL, Vol.47, No.12, pp. 2709-2719, 2023, DOI:10.32604/biocell.2023.044295 - 27 December 2023

Abstract Introduction: Helicobacter pylori is a risk factor for the development of peptic ulcers with autophagy dysfunction. Omeprazole was widely known as the first-line regimen for H. pylori-associated gastritis. Objectives: The objective of this work was to assess the role of omeprazole on cell pyroptosis and autophagy. Methods: The clinical samples were collected. Quantitative polymerase chain reaction, western blotting, enzyme linked immunosorbent assay, and immunofluorescence (IF) analysis were conducted to reveal the mechanism of omeprazole on cell pyroptosis and autophagy. Results: The results revealed that omeprazole could decrease cell pyroptosis, which was attributed to the downregulation of cleaved caspase-1 expression,… More >

WEI LU^1,*, KAN GUO², DIANMEI XI¹, ZHAOXIA XIA¹

BIOCELL, Vol.47, No.9, pp. 1995-2008, 2023, DOI:10.32604/biocell.2023.024591 - 28 September 2023

Abstract Background: Mesenchymal stem cell (MSC)-derived exosomes are closely related to pyroptosis in diabetic nephropathy (DN). This study aimed to explore the protective effect of exosomal miR-30a-5p on podocyte pyroptosis in DN. Methods: Streptozotocin was used to establish the mouse model of DN. Human bone marrow MSC-derived exosomes were extracted and identified via transmission electron microscopy, nanoparticle tracking analysis, and western blotting. MiR-30a-5p mimics and non-control (NC) mimics were transfected into MSCs and podocytes, and exosomes were isolated from the MSCs. High glucose (HG)-induced podocyte model was established to determine the effect of exosomal miR-30a-5p on pyroptosis… More >

YAO LU^1,2,*, QIUYUE WANG¹, CAIHUI ZHANG¹

BIOCELL, Vol.47, No.2, pp. 393-400, 2023, DOI:10.32604/biocell.2023.024944 - 18 November 2022

Abstract Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization… More >

CHEN WANG, CHAOFAN HE, YUANYUAN GAO, KAIXIAN WANG, MENG LIANG^*

BIOCELL, Vol.47, No.1, pp. 155-164, 2023, DOI:10.32604/biocell.2023.024657 - 26 September 2022

Abstract Heat stress is an important influence on the male reproductive organs. Therefore, the effects of heat stress on genes or pathways related to the reproductive system of male mice were experimentally explored in this paper to further determine the effects of heat stimulation on mammals. Herein, models of heat-exposed mouse testicular tissue and heat-excited cells were successfully established. Many scorched vesicles were found after heat excitation of testis supporting cells, testicular mesenchymal (TM4) cells. Western blot, in situ terminal deoxynucleotide transferase dUTP Nick end labeling (TUNEL) and transmission electron microscopy showed that membrane rupture, mitochondrial damage More >

BINFEN HOU¹, LI ZHAO¹, T IANHAO ZHAO¹, MINGMING YANG¹, WANWAN ZHU¹, XIAODONG CHEN², XIQUAN KE¹, ZHENZENG MA¹, LIN GU¹, MENG WANG¹, MIN DENG^1,*

BIOCELL, Vol.47, No.1, pp. 175-186, 2023, DOI:10.32604/biocell.2022.021359 - 26 September 2022

Abstract Aim: Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported to possess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigate the effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells. Methods: Human GC cell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation, migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwell assay, and flow cytometry. Cell migration and invasion were reassessed in… More >