Haizhen Wang*, Zhenghua Tang*, Ting Li*, Menglu Liu*, Yong Li†, Baoling Xing*
Oncology Research, Vol.27, No.9, pp. 1051-1060, 2019, DOI:10.3727/096504019X15561873320465
Abstract Medroxyprogesterone (MPA) is used for the conservative treatment of endometrial cancer. Unfortunately, progesterone resistance seriously affects its therapeutic effect. The purpose of the current study was to investigate
the influence of deletion of AT-rich interactive domain 1A (ARID1A) in progesterone resistance in Ishikawa
cells. Ablation of ARID1A was conducted through the CRISPR/Cas9 technology. Acquired progesterone-resistant Ishikawa (Ishikawa-PR) cells were generated by chronic exposure of Ishikawa cells to MPA. The sensitivity
of the parental Ishikawa, Ishikawa-PR, and ARID1A-deficient cells to MPA and/or LY294002 was determined
using the Cell Counting Kit-8 (CCK-8) assay and flow cytometry analysis.… More >
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